Sequences from confirmation amplicon


Confirmation sequences for line 179H11 and hit At1g65780 are shown.
Line ID 179H11
Locus At1g65780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24465420
Primers used for PCR 19tv - 8474, show primer details
   
Sequence Name 42-K117029-0022-179-H11C-19tv
GenBank Accession LN504608 [GenBank]
Sequencing primer gene specific primer, 19tv: 5'-ACAGGGAAGACAAAGACAACAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117029-0022-179-H11C-19tv
ACCTTGACTGTGGTCCACGAATGTTTCTGTTCTTGAAGTGGCGTCGAGAGTTTTGAAACT
AGTCTCTGGATCATTGAAGATTGGTAACTATGGTCTTGGAGATGTAGTTTTGTTTGGGAA
TGATGAGAGGATGAAGATTAAAGATAGGAAAGATCTTGTTAACATCTTCATTGATGAACG
TGTTGATAAGCTTTACCCATGTTTTATGCCTTTTTACGGGTGGAAAGCTACTATCGATGG
CATGATACGTTTACTTGAGGACCCGAAAGGACAGTATAATCTATACTTGGAAAATCTGGC
ACGGGCTAACAATGTGAAAAGGAAAGACACTGGTTCAGTCTTCAAACGCAAAGGAAATGA
GCAAAATGAAAACATAGTGGAACAAGTTTCTGATACACGTCCTCAATCATTTCAGGATTA
TCTACCAGAGAAATTCAGTGACCTTCATTTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 10-K117028-0022-179-H11C-8409
GenBank Accession LN490832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117028-0022-179-H11C-8409
ATTTCTCTGGTAGATAATCCTGAAATGATTGAGGACGTGTATCAGAAACTTGTTCCACTA
TGTTTTCATTTTGCTCATTTCCTTTGCGTTTGAAGACTGAACCAGTGTCTTTCCTTTTCA
CATTGTTAGCCCGTGCCAGATTTTCCAAGTATAGATTATACTGTCCTTTCGGGTCCTCAA
GTAAACGTATCATGCCATCGATAGTAGCTTTCCACCCGTAAAAAGGCATAAAACATGGGT
AAAGCTTATCAACACGTTCATCAATGAAGATGTTAACAAGATCTTTCCTATCTTTAATCT
TCATCCTCTCATCATTCCCAAACAAAACTACATCTCCAAGACCATAGTTACCAATCTTCA
ATGATCCAGAGACTAGTTTCAAAACTCTCGACGCCACTTCAAGAACAGAAACATTCGTTG
GACCACATGTCAAGGTTCTACATTTTGCATTCAGTAATGTGAACAACAACACACTTGTTG
T

Last Updated on 06.12.2018 16:11