Sequences from confirmation amplicon


Confirmation sequences for line 180A01 and hit At1g75500 are shown.
Line ID 180A01
Locus At1g75500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28339685
Primers used for PCR HV63 - 8474, show primer details
   
Sequence Name 79-K101271-0022-180-A01C-8409
GenBank Accession LN513796 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K101271-0022-180-A01C-8409
CTTTACATTGAATATATCCTGCCGGGAAATGAATATATGAAATTGAATATATCAATAACA
AATTTGGATAATTAGTTAATGAATTATTACACGACAGAATAACAGCGAACCAAGGATTTT
ACTTGTTGGGACTGGACAACACTTCACCAACATTTGCTTCCTCCATGCAAAACTCTGTTC
CCGCCATTACCTTTCTCATGGCTGCTCTTCTCAGGCACTTACTCTACACATCTCTCCATT
TTCATTCTCTTTTGATTTATAAAAATAAATAACAATATTTTGGACAGCTTGGATATACAA
GAACCACTTTAATAATGTCCATTTGACTTTTCAACGTAAAAGAAACGGATAATAATACAC
GTAGACNATATTTAATGCCGTTTCCATTTGCTTACAAAATAGTGATAATCATAGTAGTAA
CAACAATGTAACATACTGTTTATGTACTGTATGTGAAAAAAACAATAATTCAGGATTGAG
AAAGTAAGAATAAACAGAAGAGACGGTATCTCCAAAATCTTAGGAACAGCTCTTTGTGTC
GCCGGAGCTTCCGTCATCACCCTCTATAAAGGTCCAACCATCTACACTCCGGCTAGCCAC
CTCCACGCTCATCTACTCACCACAAACTCCGCCGTCTTAGCGCCGCTNGGAAACGCCGCG
CCTAANAACTGGACCCTTGGTTGCATCTACCTAATCGGTCACTGTCTCTCGTGGTCAGGC
TGGCTTGTTTTCCAAGCTCCGGTTCTNAAGTCTTATCCAGCGAGGCTCTCGGTTACGTCT
TACACTTGTTTCTTCGGA
   
Sequence Name 79-K101277-0022-180-A01C-HV63
GenBank Accession LN513797 [GenBank]
Sequencing primer gene specific primer, HV63: 5'-AAGAACTGAATGATTCCGAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K101277-0022-180-A01C-HV63
ATAAGACTTAGAACCGGAGCTTGGAAAACAAGCCAGCCTGACCACGAGAGACAGTGACCG
ATTAGGTAGATGCAACCAAGGGTCCAGTTTTTAGGCGCGGCGTTTCCTAGCGGCGCTAAG
ACGGCGGAGTTTGTGGTGAGTAGATGAGCGTGGAGGTGGCTAGCCGGAGTGTAGATGGTT
GGACCTTTATAGAGGGTGATGACGGAAGCTCCGGCGACACAAAGAGCTGTTCCTAAGATT
TTGGAGATACCGTCTCTTCTGTTTATTCTTACTTTCTCAATCCTGAATTATTGTTTTTTT
CACATACAGTACATAAACAGTATGTTACATTGTTGTTACTACTATGATTATCACTATTTT
GTAAGCAAATGGAAACGGCATTAAATATTGTCTACGTGTATTATTATCCGTTTCTTTTAC
GTTGAAAAGTCAAATGGACATTATTAAAGTGGTTCTTGTATATCCAAGCTGTCCAAAATA
TTGTTATTTATTTTTATAAATCAAAAGAGAATGAAAATGGAGAGATGTGTAGAGTAAGTG
CCTGAGAAGAGCAGCCATGAGAAAGGTAATGGCGGGAACAGAGTTTTGCATGGAGGAAGC
AAATGTTGGTGAAGTGTTGTCCAGTCCCAACAAGTAAAATCCTTGGTTCGCTGTTATTCT
GTCGTGTAATAATTCATTAACTAATTATCCAAATTTGTTATTGATATATTCAATTTCATA
TATTCATTTCCCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTNCATATGGAGAANAAGAAAGAGTAATTACCATTTTT
TT

Last Updated on 06.12.2018 16:11