Sequences from confirmation amplicon


Confirmation sequences for line 180A11 and hit At5g06780 are shown.
Line ID 180A11
Locus At5g06780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2094045
Primers used for PCR BU02 - 8474, show primer details
   
Sequence Name 56-K023016-022-180-A11C-8409
GenBank Accession LN508979 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K023016-022-180-A11C-8409
CTGATCCATGTAGATTTCCCGGACATGAAGCCATTTACATTTTCTATGCATCTTTAGTAA
TGGAAGAACATTTACATGTAGATTATTTTCATATATGAACAAAATCATTTTCTGGGATCC
CTTAAAGCAAAACAGAAGAAAAAGAAATATTGCTAGTCATTTTTCCTAGGGGTGATGGTG
TCATACATACTCACCCCAGCAGCATTGGAAGCTCATGCGAATACGGTTCTTCTCCATCTG
GTTCACCAGCACAAATTATGTTGTTGTTAGTGATCTTAGTGCATGACAAATAGAAATAAA
CAAAGGCCGCTGTTCACATTTATTACCGCTTTCGTAATCAGAAGCATCCGTAAGCCTTGC
AATGGCAGCAATAAGCGCCTGTTCATGTTCCTATATCATCCAATAACAACAACACCAAGT
TTCACTTCACTGTTTCTTTCAATCGATATCTCGTCACAAAGTTCTTATCAATTATCATAT
CGCTCACCTTGAGTAGCTTCT
   
Sequence Name 57-K023016-022-180-A11C-BU02
GenBank Accession LN509287 [GenBank]
Sequencing primer gene specific primer, BU02: 5'-GATAAGGCCAAGAAGCTACTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K023016-022-180-A11C-BU02
AGCGATATGATAATTGATAAGAACTTTGTGACGAGATATCGATTGAAAGAAACAGTGAAG
TGAAACTTGGTGTTGTTGTTATTGGATGATATAGGAACATGAACAGGCGCTTATTGCTGC
CATTGCAAGGCTTACGGATGCTTCTGATTACGAAAGCGGTAATAAATGTGAACAGCGGCC
TTTGTTTATTTCTATTTGTCATGCACTAAGATCACTAACAACAACATAATTTGTGCTGGT
GAACCAGATGGAGAAGAACCGTATTCGCATGAGCTTCCAATGCTGCTGGGGTGAGTATGT
ATGACACCATCACCCCTAGGAAAAATGACTAGCAATATTTCTTTTTCTTCTGTTTTGCTT
TAAGGGATCCCAGAAAATGATTTTGTTCATATATGAAAATAATCTACATGTAAATGTTCT
TCCATTACTAAAGATGCATAGAAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11