Sequences from confirmation amplicon


Confirmation sequences for line 180F05 and hit At5g53480 are shown.
Line ID 180F05
Locus At5g53480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21715402
Primers used for PCR CK30 - 8474, show primer details
   
Sequence Name 16-K017884-022-180-F05C-CK30
GenBank Accession LN493221 [GenBank]
Sequencing primer gene specific primer, CK30: 5'-AAGGCTGTAAGAGAAGATGACGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K017884-022-180-F05C-CK30
CTCTACAGGCAATTGAGTTCTGGAGTTCTATTTGTGACGAAGAGATTGACATCTTAGAAG
AATATGGTGGTGAGTTCGCTGGGGATTCTGATGTTCCATGCTTTTATTTTACTAAGCAGG
CTCTCCCTGGTCTTGTGCCTCTACTGCTGGAGACTCTTCTGAAGCAAGAAGAAGATCAAG
ATTTGGATGAAGGGGCTTGGAATATTGCAATGGCAGGTGGGACATGCCTCGGTTTGGTTG
CTAGGGCAGTTGGAGATGACATTGTTCCACATGTCATGCCGTTTATTGAAAAGAAAATAT
CAAAGCCTGATTGGAAAGAGCGAGAAGCTGCAACTTATGCTTTTGGTTCCATTTTGGAAG
GCCCTTCTGCCGATAAGTTGATGGCAATTGTTAACGCACCGTTAACATTTATGCTCAATG
CTCTAACAAATGACCCAAGCAACCATGTGAAAAACACAACCGCATGGACCCTTGGCCGGA
TATTTAAGTTCCTTCTTGGTTCACCAATCAAA
   
Sequence Name 17-K017884-022-180-F05C-8409
GenBank Accession LN493661 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K017884-022-180-F05C-8409
TAGATTTCCCGGACATGAAGCCATTTATTTTTGTACTGTGATTATCTGCTGGCAGTTTGC
CTGATTAATAATAGGTGTCTCGATTGTTGAACCATGAAGGAACTCAAATATCCGACCAAG
GGTCCATGCGGTTGTGTCTTTCACATGGTTGCTTGGGTCATTTGTTAGAGCATTGAGCAT
AAATGTTAACGCTGCGTTAACAATTGCCATCAACTTATCGGCAGAAGGGCCTTCCAAAAT
GGAACCAAAAGCATAAGTTGCAGCTTCTCGCTCTCTCCAATCAGGCTTTGATATTTTCTC
TTCAATAAACGGCATGACATGTGGAACAATGTCATCTCCAACTGCCCTAGCAACCAAACC
GAGGCATGTCCCACCTGCCATTGCAATATTCCAAGCCCCTTCATCCAAATCTTGATCTTC
TTCTTGCTTCAGAAGAGTCTCCAGCAGTAGAGGCACAAGACCAGGGAGAGCCTGCTTAGT
AAAATAAAAGCATGGAACATCAGAATCCCCAGCGAACTCACCACCATATTCTTCTAAGAT
GTCAATCTCTTCGTCACAAATAGAACTCCAGAACTCAATTGCCTGTAGAGCAACAGACTC
GTCATCTTCT

Last Updated on 06.12.2018 16:11