Sequences from confirmation amplicon


Confirmation sequences for line 180G04 and hit At1g33612 are shown.
Line ID 180G04
Locus At1g33612 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12192797
Primers used for PCR AY12 - 8474, show primer details
   
Sequence Name 33-K017551-022-180-G04C-AY12
GenBank Accession LN500796 [GenBank]
Sequencing primer gene specific primer, AY12: 5'-AATCTTCTAACTGGTCCATTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K017551-022-180-G04C-AY12
TATTGAAAAGCATTAACGGCATTGAATCTCTGGATTTGTCGTACAACAAGTTTCACCTGA
AAACGATTCCAAAATGGATGATATCGTCACCATCCATCTACTCATTGAAGTTGGCAAAAT
GTGGGCTGAAGATAAGCTTAGATGATTGGAAGCTAGCAGGGACCTACTACTATGATTCCA
TCGATCTGTCTGAAAACGAGATCTCAGGGAGTCCGGCTAAGTTCCTCAGCCAGATGAAGT
ATCTGATGGAGTTCCGAGCGGCAGGGAACAAACTCCGATTCGATTTGGGGAAGCTGACGT
TTGTGAGGACTCTTGAAACCTTGGATTTGTCAAGGAACTTGATATTCGGGAGAGTGCTGG
CAACATTTGCTGGACTGAAGACAATGAACGTGAGTCAAAACCATCTTTGCGGAAAACTTC
CGGTGACAAAGTTCCCGGCCAGTGCGTTTGCGGGTAACGACTGTCTTTGTGGCTCTCCAC
TTTCTCCTTGTAAGTGTAGTGGCAAGAAAACTATAATTAGAATCTGTATCTAATGTGTCG
ACAGTTGGGTCCAAAAAATATTTGTACTAACTCTTATTATTATAAGGGATTCTCTTATTC
AAGAGTTACGTGTGGCATCTGTAGCAATGAGTATGATGGTCAATATGGAGAAAAA
   
Sequence Name 34-K017551-022-180-G04C-8409
GenBank Accession LN501220 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K017551-022-180-G04C-8409
ATGCCACACGTAACTCTTGAATAAGAGAATCCCTTATAATAATAAGGTTAGTACAAATAT
TTTTTGGACCCAACTGTCGACACATTAGATACAGATACTAATTATAGTTTTCTTGCCACT
ACACTTTACAAGGAGAAAGTGGAGAGCCACAAAGACAGTCGTTACCCGCAAACGCACTGG
CCGGGAACTTTGTCACCGGAAGTTTTCCGCAAAGATGGTTTTGACTCACGTTCATTGTCT
TCAGTCCAGCAAATGTTGCCAGCACTCTCCCGAATATCAAGTTCCTTGACAAATCCAAGG
TTTCAAGAGTCCTCACAAACGTCAGCTTCCCCAAATCGAATCGGAGTTTGTTCCCTGCCG
CTCGGAACTCCATCAGATACTTCATCTGGCTGAGGAACTTAGCCGGACTCCCTGAGATCT
CGTTTTCAGACAGATCGATGGAATCATAGTAGTAGGTCCCTGCTAGCTTCCAATCATCTA
AGCTTATCTTCAGCCCACATTTTGCCAACTTCAATGAGTAGATGGATGGTGACGATATCA
TCCCATTTGGATCGTTTTCAGGTGAAACTTGTTGTACGACAAATC

Last Updated on 06.12.2018 16:11