Sequences from confirmation amplicon


Confirmation sequences for line 180G04 and hit At3g23840 are shown.
Line ID 180G04
Locus At3g23840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8612414
Primers used for PCR 13jr - 8474, show primer details
   
Sequence Name 24-K113214-0022-180-G04C-8409
GenBank Accession LN497069 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K113214-0022-180-G04C-8409
ATTTACAATTGAACATTACAATAAACCGATTCCGGCGATTTCCCCATAACTTTCGCCTCA
TAAAAATCAACTTCACTCATATCCACAAACGTTAAATTCGCTCCGTAAACAATAAAATCC
GAAACATCATCAACAATCTCATCGATCACAACCCTCTCATCTTTCGCTTCACCGATCGAT
TTCACAATCTCCTCCAAACTCGCCTCCGCCACCGAGAAATCGACGTGAATGGAACTTATC
ATCTGACCATTTCTCACCGCTCTAGGCTTCAACTTCTTCGGATCCGATCTGATAACCGTA
ATCGTCACCGGAGCTGATTCTCCCCTCACCGTCGCAACACATTTCCATATGATTCCAGTG
AGAATCTCGAATTCGCCGTCTCCGTTCACCGGAAAATGTGTTTTTAAATCGTTAACCGTT
AAATTGAAGGAGAATGTTGTCATTTTGCTGTTATTCGGAGCGACCCAGAGATCTCCAACC
AGGTCAACCTGTTTGACTGAATCCGGTTTTTTGAATGTTGAGGTCGGGTTTTGAAAGTCT
CTCTCTGTGACAGAGGTTTTGGGGCAGTAGATCTTCCCACCAGCAAAGGCTTGAGCCCAT
AAGTTGAGAAAATGA
   
Sequence Name 24-K113215-0022-180-G04C-13jr
GenBank Accession LN497070 [GenBank]
Sequencing primer gene specific primer, 13jr: 5'-CTCATTTCTTCAACTTATGGGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K113215-0022-180-G04C-13jr
CAAACCTCTGTCCAGAGAGAGACTTTCAAACCCGACCTCAACATTCAAAAAACCGGATTC
AGTCAAACAGGTTGACCTGGTTGGAGATCTCTGGGTCGCTCCGAATAACAGCAAAATGAC
AACATTCTCCTTCAATTTAACGGTTAACGATTTAAAAACACATTTTCCGGTGAACGGAGA
CGGCGAATTCGAGATTCTCACTGGAATCATATGGAAATGTGTTGCGACGGTGAGGGGAGA
ATCAGCTCCGGTGACGATTACGGTTATCAGATCGGATCCGAAGAAGTTGAAGCCTAGAGC
GGTGAGAAATGGTCAGATGATAAGTTCCATTCACGTCGATTTCTCGGTGGCGGAGGCGAG
TTTGGAGGAGATTGTGAAATCGATCGGTGAAGCGAAAGATGAGAGGGTTGTGATCGATGA
GATTGTTGATGATGTTTCGGATTTTATTGTTTACGGAGCGAATTTAACGTTTGTGGATAT
GAGTGAAGTTGATTTTTATGAGGCGAAAGTTATGGGGAAATCGCCGGAATCGGTTTATTG
TAATGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGGTTA

Last Updated on 06.12.2018 16:11