Sequences from confirmation amplicon


Confirmation sequences for line 181H12 and hit At4g18880 are shown.
Line ID 181H12
Locus At4g18880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10347926
Primers used for PCR ER04 - 8474, show primer details
   
Sequence Name 25-K025267-022-181-H12C-8409
GenBank Accession LN497281 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K025267-022-181-H12C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCTGTAGAAAAACTGCTGCCA
GAAGCCATCATTCGCTCCTGCTACTGGAGGAGGAGGAGGAGCAGCAACAGTGTTCTGTTC
TTTCGAACCATCGGGCTCACAGTTCATATCGATGATCCTTGGAGAAGGAGGAGATTTGAG
ACGTGAATCGACACTTAACTGTATGCAAGAAAGAGGAGGGCTCTCTGGAAAAGTAGATGA
TTCATCCACATCAAGTGTCATCATACTTCTTGATTGTAACATACTCTCACAAGAATCCGA
TACAAGATTCTCCCAAATCGCTATCGATGACTCTAACTGTTCCACTTGATGCTCTCTTGT
GTG
   
Sequence Name 26-K025267-022-181-H12C-ER04
GenBank Accession LN497757 [GenBank]
Sequencing primer gene specific primer, ER04: 5'-CTTCTTCACACACAAGAGAGCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K025267-022-181-H12C-ER04
TGGAACAGTTAGAGTCATCGATAGCGATTTGGGAGAATCTTGTATCGGATTCTTGTGAGA
GTATGTTACAATCAAGAAGTATGATGACACTTGATGTGGATGAATCATCTACTTTTCCAG
AGAGCCCTCCTCTTTCTTGCATACAGTTAAGTGTCGATTCACGTCTCAAATCTCCTCCTT
CTCCAAGGATCATCGATATGAACTGTGAGCCCGATGGTTCGAAAGAACAGAACACTGTTG
CTGCTCCTCCTCCTCCTCCAGTAGCAGGAGCGAATGATGGCTTCTGGCAGCAGTTTTTCT
ACAGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11