Genotyping and primer details


Genotyping and primer details for 181H12 - At4g18880
Line ID 181H12
Locus At4g18880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10347926 of line 181H12 Chr4:10347926
   
Insertion allele amplicon 8474 - ER04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ER04: 5'-CTTCTTCACACACAAGAGAGCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10348256
   
wt amplicon (over InsPos) 39hs - ER04
wt amplicon start Chr4:10347363
wt amplicon end Chr4:10348256
wt amplicon size 894 bp
Locus specific primer ER04: 5'-CTTCTTCACACACAAGAGAGCATC-3'
2nd locus specific primer 39hs: 5'-TTGGCTTTCTGCTTTCTGTAAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11