Sequences from confirmation amplicon


Confirmation sequences for line 183G07 and hit At5g01770 are shown.
Line ID 183G07
Locus At5g01770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:297202
Primers used for PCR EP78 - 8474, show primer details
   
Sequence Name 56-K103246-0022-183-G07C-EP78
GenBank Accession LN509071 [GenBank]
Sequencing primer gene specific primer, EP78: 5'-TGCATAACCAACTTACCTGTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K103246-0022-183-G07C-EP78
GAGACGACTAGGCCAGCCGAGGAGTGTGAGATGCTGATGCTGCTTCACCTGGTCGGCCAT
TGGACTTGGAGGGTTTCGCAACAATTTGTTCAATCCCAATAACAGAAAGCACACGCCGCC
CAAGGCTTGCAATACGTGGAGATGGATCTTTAGCTAAGGTAAACATAGCTAGAACACTCT
GCGAATAGATAGCATTATCCAGCGGTCTTGGCTGATGGACAACTCCNTTGCTGACACCAT
CATGGATGATTCCAACATCAGAATGCTATCTATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 08-K103247-0022-183-G07C-8409
GenBank Accession LN489693 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K103247-0022-183-G07C-8409
GGAATGAGCCTTTACATTGAATAGATGCATTCTGATGTTGGAATCATCCATGATGGTGTC
AGCAATGGAGTTGTCCATCAGCCAAGACCGCTGGATAATGCTATCTATTCGCAGAGTGTT
CTAGCTATGTTTACCTTAGCTAAAGATCCATCTCCACGTATTGCAAGCCTTGGGCGGCGT
GTGCTTTCTGTTATTGGGATTGAACAAATTGTTGCGAAACCCTCCAAGTCCAATGGCCGA
CCAGGTGAAGCAGCATCAGCATCTCACACTCCTCTGGCTGGCCTAGTTCGCTCATCCTCT
TGGTTTGACATGCATACNGGTTAGTTGGTTATGC

Last Updated on 06.12.2018 16:11