Sequences from confirmation amplicon


Confirmation sequences for line 184B02 and hit At5g23510 are shown.
Line ID 184B02
Locus At5g23510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7928030
Primers used for PCR KT29 - 8474, show primer details
   
Sequence Name 36-K100814-0022-184-B02C-8409
GenBank Accession LN502139 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K100814-0022-184-B02C-8409
CCATTTACATTGAATATATCCTGTCAATTGTAAGCACATCTAACACAACTGTACTTTCCA
TATCAAGATTCCAGTATACCACAAAATCATAATCCTAGCTTTCCACAGAGAAGAACAACT
CTAACATATGATCTCATTCATATAAAAAGATATTAAACCTTCCGGTTCCGGTCATCCAAA
GGCTGAACTTCAATAGCAAGACATAAACCAACATCATCAGCAGTGACAAGGTAATTTGGC
TTCTTTGCTCCTGCATTAGAATTTCATTAACGACTATATTCTTATTTCCATATAAAGAAA
ATAAGAAACAGAAATCAGCAGGAGGGGAATGTACCATCAATGTAATTCACAGATCCATCT
TCTAGATGACATACCCACTGAAAATGGAAGAACAGAAGAAGATAGAGCAATTCAACATAA
GTAAGAAAGG
   
Sequence Name 36-K100826-0022-184-B02C-KT29
GenBank Accession LN502141 [GenBank]
Sequencing primer gene specific primer, KT29: 5'-CAACAGAATCGCTTCCTTTCTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K100826-0022-184-B02C-KT29
TTTCAGTGGGTATGTCATCTAGAAGATGGATCTGTGAATTACATTGATGGTACATTCCCC
TCCTGCTGATTTCTGTTTCTTATTTTCTTTATATGGAAATAAGAATATAGTCGTTAATGA
AATTCTAATGCAGGAGCAAAGAAGCCAAATTACCTTGTCACTGCTGATGATGTTGGTTTA
TGTCTTGCTATTGAAGTTCAGCCTTTGGATGACCGGAACCGGAAGGTTTAATATCTTTTT
ATATGAATGAGATCATATGTTAGAGTTGTTCTTCTCTGTGGAAAGCTAGGATTATGATTT
TGTGGTATACTGGAATCTTGATATGGAAAGTACAGTTGTGTTAGATGTGCTTACAATTGA
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCC
Primers used for PCR KT30 - 8474, show primer details
   
Sequence Name 37-K100814-0022-184-B02C-OriL
GenBank Accession LN502535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K100814-0022-184-B02C-OriL
CCATTTACATTGAATATATCCTGATATTATTATTTAATGTTTTTCAGTTTTTTCATGTTG
CTACTTTCTGATTTCAGGGAGAGCTTGTGAAGGTTTTTGCCAATGATAATCGCAAGATCG
CTTGCCGTAAGTTAATCTATTTACTTAGCTAGAGAATCTCCCTCTTGAGTTCCTATAAAG
CTAGACCACTTGTCAGTTTACGTCTCGATGTTAAGTCTTTTGTGCTCGCTGAGCTGTTTC
TGGTTGGTTGTGCAAGAGATTGGTTCCCTTAAATATGTAGCATTCTTTGAGAACCTAATA
GTATTTATTGTCAGACTTCTATCTAACAGTAATCCTCTTTTTATTTTCACAGATCCNGAG
ATGCAGAGCAATATAGACNAGACTCTTCATACTGGTCATGCTTCATACAAAGTTTCTCTT
GCGGTATCACATTTTCTGTTTCAACTCGTATATNTTGGCTATGTTTTCCTTGGATTAATC
CTTCACATGCATGTGGCTNTGGATAGATTGGTTTCGTGCATATATGGGAAGCAGCTC
   
Sequence Name 37-K100826-0022-184-B02C-KT30
GenBank Accession LN502537 [GenBank]
Sequencing primer gene specific primer, KT30: 5'-CAATAGATAACGTAGCTGCTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K100826-0022-184-B02C-KT30
AAGCCCATGCATGTGAAGGATTAATCCAAGGAAAACATAGCCAAAATATACGAGTTGAAA
CAGAAAATGTGATACCGCAAGAGAAACTTTGTATGAAGCATGACCAGTATGAAGAGTCTT
GTCTATATTGCTCTGCATCTCTGGATCTGTGAAAATAAAAAGAGGATTACTGTTAGATAG
AAGTCTGACAATAAATACTATTAGGTTCTCAAAGAATGCTACATATTTAAGGGAACCAAT
CTCTTGCACAACCAACCAGAAACAGCTCAGCGAGCACAAAAGACTTAACATCGAGACGTA
AACTGACAAGTGGTCTAGCTTTATAGGAACTCAAGAGGGAGATTCTCTAGCTAAGTAAAT
AGATTAACTTACGGCAAGCGATCTTGCGATTATCATTGGCAAAAACCTTCACAAGCTCTC
CCTGAAATCAGAAAGTAGCAACATGAAAAAACTGAAAAACATTAAATAATAATATCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCG

Last Updated on 06.12.2018 16:11