Sequences from confirmation amplicon


Confirmation sequences for line 184D06 and hit At4g31550 are shown.
Line ID 184D06
Locus At4g31550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15290220
Primers used for PCR IS05 - 8474, show primer details
   
Sequence Name 27-K015964-022-184-D06C-8409
GenBank Accession LN498159 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K015964-022-184-D06C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATGTACTGCACTTGTAGTAACCACTGGA
TCGCAAAAAACAAATAAACACCGTTAGATCGCGATTAAATTAAATACCAACGGTGGATAT
TAGACGGGTGTAGGATATTTACCGTGGGTGTGGTGAGCCCTTGATCGGTTTTTGTCCGTA
CTTCCTCCACGAATATTCGTCCGGTGGAATATCGGCGATCTTTGCACTTATCGCCGGTAC
TCTCACGGTTCTCTTCATCCGATTTTTCCTGCTTCATTAAAAATCAAATAGAAAATATTA
ATTAACGGAAAAAACATTGGGTTCCAAAAGTCAACGGTCAAAACTTGAG
   
Sequence Name 28-K015964-022-184-D06C-IS05
GenBank Accession LN498625 [GenBank]
Sequencing primer gene specific primer, IS05: 5'-ACTCAAGTTTTGACCGTTGACTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K015964-022-184-D06C-is05
CCAATGTTTTTTCCGTTAATTAATATTTTCTATTTGATTTTTAATGAAGCAGGAAAAATC
GGATGAAGAGAACCGTGAGAGTACCGGCGATAAGTGCAAAGATCGCCGATATTCCACCGG
ACGAATATTCGTGGAGGAAGTACGGACAAAAACCGATCAAGGGCTCACCACACCCACGGT
AAATATCCTACACCCGTCTAATATCCACCGTTGGTATTTAATTTAATCGCGATCTAACGG
TGTTTATTTGTTTTTTGCGATCCAGTGGTTACTACAAGTGCAGTACATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11