Genotyping and primer details


Genotyping and primer details for 184D06 - At4g31550
Line ID 184D06
Locus At4g31550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15290220 of line 184D06 Chr4:15290220
   
Insertion allele amplicon 8474 - IS05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS05: 5'-ACTCAAGTTTTGACCGTTGACTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15290540
   
wt amplicon (over InsPos) 15ee - IS05
wt amplicon start Chr4:15289641
wt amplicon end Chr4:15290540
wt amplicon size 900 bp
Locus specific primer IS05: 5'-ACTCAAGTTTTGACCGTTGACTTT-3'
2nd locus specific primer 15ee: 5'-ACCCTTAACTTGTCTTTCGGATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11