Sequences from confirmation amplicon


Confirmation sequences for line 185D08 and hit At1g52100 are shown.
Line ID 185D08
Locus At1g52100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19384091
Primers used for PCR BL70 - 8474, show primer details
   
Sequence Name 49-K105459-0022-185-D08C-8409
GenBank Accession LN506841 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K105459-0022-185-D08C-8409
ATTTACATTGAATATTGACCATCATACCCAACCTTACAATTGTGTTGTTCTTACATCGTT
AACTTTCAAAACATCAAAAGGAAGAGCCTCTCCAGCGATTGGAAAAGTGACTGGTAGCAA
ATTCAAGCTGGAGAGACAAGGTGATGCTATTGTTGGGTTCCATGGACGGGTAGGCTCTTG
TATTGATGGTATCGGGGTGTATTACGCTCCACTTCCACCTTCTCCTCCTCCTCCAGAGAA
ACTCCAAGGCCAAGGTGGTGACGGTGGAGATTCTTGGGACGATGGTGCTTTCAAAAATGT
GAAGAAGATATATGTAGGACAAGGCGATGTTGGGATTGCTGCAGTCAAATTTGAGTACGA
GACTTACACTAGTGAGGTGATTCTGGCAGAAAGACATGGAAAGGAAACATTGCTCGGATA
CGAAGAGGTTCAGTTTCTCTTTACCCTAACATTTTATCTCAAAAAACCAATTGAAAATGA
ATGGATATTATATTTACATCTTTTATATTAAGCATGATCTCTTGCATTTCTCAAATGTGA
AATTCCTAACATTTAGTTTTCATCTACTACACAGTTTGAACTGGACTATCCGAGTGAGTA
CATTACA
   
Sequence Name 33-K105458-0022-185-D08C-BL70
GenBank Accession LN500995 [GenBank]
Sequencing primer gene specific primer, BL70: 5'-CCTTCCACAGCTGTAATGTACTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K105458-0022-185-D08C-BL70
ATGAAACTAAATGTTAGGAATTTCACATTTGAGAAATGCAAGAGATCATGCTTAATATAA
AAGATGTAAATATAATATCCATTCATTTTCAATTGGTTTTTTGAGATAAAATGTTAGGGT
AAAGAGAAACTGAACCTCTTCGTATCCGAGCAATGTTTCCTTTCCATGTCTTTCTGCCAG
AATCACCTCACTAGTGTAAGTCTCGTACTCAAATTTGACTGCAGCAATCCCAACATCGCC
TTGTCCTACATATATCTTCTTCACATTTTTGAAAGCACCATCGTCCCAAGAATCTCCACC
GTCACCACCTTGGCCTTGGAGTTTCTCTGGAGGAGGAGGAGAAGGTGGAAGTGGAGCGTA
ATACACCCCGATACCATCAATACAAGAGCCTACCCGTCCATGGAACCCAACAATAGCATC
ACCTTGTCTCTCCAGCTTGAATTTGCTACCAGTCACTTTTCCAATCGCTGGAGAGGCTCT
TCCTTTTGATGTTTTGAAAGTTAACGATGTAAGAACAACACAATTGTAAGGTTGGGTATG
ATGGTCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTC

Last Updated on 06.12.2018 16:11