Sequences from confirmation amplicon


Confirmation sequences for line 186B08 and hit At1g53990 are shown.
Line ID 186B08
Locus At1g53990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20151475
Primers used for PCR KS67 - 8474, show primer details
   
Sequence Name 35-K101564-0022-186-B08C-8409
GenBank Accession LN501769 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101564-0022-186-B08C-8409
GCCATTTACAATTGAATATATCCTGCCGGCTAAAGCTCCGGCGCCAGCAGAAGCAAAACT
TACTCCATAGGTAAATTGATTGTTACCGTTGTTTGGTTGTAGATTTGGCGGGATCGACGG
CAACCATGCCTTTTCGGCTTCCAGGTCAGCAATTAGTGAATAAAAAAATGTTAAACATTC
CTAGGAAATAAAAATAACAAAGCAGAGTAGTTTTGTTATGTTAACGATGAAGTCGGGAAT
CAAACGTCCATCGGATAATCTTCCGGTGGGAAACTTGAAATTGGTTTGACCGTACGGCCA
GATATTAGACCGGAAACTTGAGACAGTATTGATGTAGTTGTTGTTTCCGGCATCAAAAAG
AGAATCTCCGAACACGAACAAAGCAGCTTGATTTGTTACCAGATTATTATTGTCTATGCA
GTTGATTGAGCCGATCGACAAGATTATTGTGTAGACGAAGAAGATGATCAAAACTAATCG
AACCATGTTTAATCGTTAACGTTTATGTTCTGTCCACAAGCTAGCACTTGTTGAATATAT
ATATTTATAATTTTTTTTATAACAGTTTTGTTCTAACAGTTTTTTTTTTCTTGGTTATAG
AGTATAGACATTCTTAATTATGCATGGTCAAGTTTGTTGTTCTAAAGCAAAATTTTCCGA
TGCACGGCTTTAATCAAAATCGA
   
Sequence Name 35-K101577-0022-186-B08C-KS67
GenBank Accession LN501770 [GenBank]
Sequencing primer gene specific primer, KS67: 5'-ATATCGATTTTGATTAAAGCCGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101577-0022-186-B08C-KS67
CTTGACCATGCATAATTAAGAATGTCTATACTCTATAACCAAGAAAAAAAAAACTGTTAG
AACAAAACTGTTATAAAAAAAATTATAAATATATATATTCAACAAGTGCTAGCTTGTGGA
CAGAACATAAACGTTAACGATTAAACATGGTTCGATTAGTTTTGATCATCTTCTTCGTCT
ACACAATAATCTTGTCGATCGGCTCAATCAACTGCATAGACAATAATAATCTGGTAACAA
ATCAAGCTGCTTTGTTCGTGTTCGGAGATTCTCTTTTTGATGCCGGAAACAACAACTACT
TCAATACTGTCTCAAGTTTCCGGTCTAATATCTGGCCGTACGGTCAAACCAATTTCAAGT
TTCCCACCGGAAGATTATCCAATGGACGTTTGATTCCCGACTTCATCGTTAACATAACAA
AACTACTCTGCTTTGTTATTTTTATTTCCAAGGAATGTTTAACATTTTTTTATTCACTAA
TTGCTGACCTGGAAGCCGAAAAGGCATGGTTGCCGTCGATCCCGCCAAATCTACAACCAA
ACAACGGTAACAATCAATTTACCTATGGAGTAAGTTTTGCTTCTGCTGGCGCCGGAGCTT
TAGCCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTA

Last Updated on 06.12.2018 16:11