Sequences from confirmation amplicon


Confirmation sequences for line 186E10 and hit At4g02690 are shown.
Line ID 186E10
Locus At4g02690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1186098
Primers used for PCR CD59 - 8474, show primer details
   
Sequence Name 75-K104358-0022-186-E10C-CD59
GenBank Accession LN513237 [GenBank]
Sequencing primer gene specific primer, CD59: 5'-GTGGTTGATCAAGTCATACCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K104358-0022-186-E10C-CD59
CCACACAAAGCAGAGCCAAGTGAAATCCCCAGAGCAAGTAATTCACCGGATGTTTCTGAT
GGTAATAGTACAACGGACACAACACTATACATCGGAGAGAAACACAAATCCAAGTTTCAG
CTTTTGAGATTATTACCAAACTTCGAAAAAGATTGAAACTTTTGATTTGTAAAACAAGTG
TGCGTGATGATTGTACCTATGAGAGGTGTGATGATGATGACTATGTAAAGAGCTAATCCA
AGTCCGGTGGTGGCGAAGAACAGAGCGATTGGACGGACGGTGACCACCGTGGCAGCGACA
GCGACGGTGGCTAGAAGCTGAAAGGCGATTATAGAGTAGACCTTGCGTATAAATCCCCAC
CGGAGCTCGGGATTTTCATGCATCGCTGGATTTTCAGGCGTTACCGGGATTTTCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGGTTATT
   
Sequence Name 83-K104359-0022-186-E10C-8409
GenBank Accession LN514309 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K104359-0022-186-E10C-8409
GCATTTACAATTGAATATATCCTGAAAATCCCGGTAACGCCTGAAAATCCAGCGATGCAT
GAAAATCCCGAGCTCCGGTGGGGATTTATACGCAAGGTCTACTCTATAATCGCCTTTCAG
CTTCTAGCCACCGTCGCTGTCGCTGCCACGGTGGTCACCGTCCGTCCAATCGCTCTGTTC
TTCGCCACCACCGGACTTGGATTAGCTCTTTACATAGTCATCATCATCACACCTCTCATA
GGTACAATCATCACGCACACTTGTTTTACAAATCAAAAGTTTCAATCTTTTTCGAAGTTT
GGTAATAATCTCAAAAGCTGAAACTTGGATTTGTGTTTCTCTCCGATGTATAGTGTTGTG
TCCGTTGTACTATTACCATCAGAAACATCCGGTGAATTACTTGCTCTTGGGGATTTTCAC
TTTGGCTCTGGCTTTTGTTGTTGGATTAACATGTGCCTTCACCAATGGTATGACTTGATC
AACC

Last Updated on 06.12.2018 16:11