Sequences from confirmation amplicon


Confirmation sequences for line 187A10 and hit At4g23580 are shown.
Line ID 187A10
Locus At4g23580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12304668
Primers used for PCR BL71 - 8474, show primer details
   
Sequence Name 74-K105064-0022-187-A10C-BL71
GenBank Accession LN513075 [GenBank]
Sequencing primer gene specific primer, BL71: 5'-AACGCTTTGTCTAGCGTTATGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K105064-0022-187-A10C-BL71
GCTCCAGCATGCGGGTGGGCCGTGTGTTTCCATCTGCTTGTACTCTTGATGGGAGAATAT
ATGTTACGGGAGGCTGCGACAATCTCGATACAATGAACTGGATGGAGATTTTTGATACGA
AGACTCAAACTTGGGAGTTTCTACAGATCCCTAGCGAGGAGATATGCAAAGGCTCTGAGT
ACTTAAGCGTAAGTTATCAAGGAACCGTCTATGTGAAGTCTGATGAAAAAGATGTGACTT
ACAAGAAAAAGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAT
   
Sequence Name 58-K105065-0022-187-A10C-8409
GenBank Accession LN509701 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K105065-0022-187-A10C-8409
TTCTTTTCTTGTAGTCACATCTTTTTCTCAGACTTCACATAGACGGTTCCTTGATAACTT
ACGCTTAAGTACTCAGAGCCTTTGCATATCTCCTCGCTAGGGATCTGTAGAAACTCCCAA
GTTTGAGTCTTCGTATCAAAAATCTCCATCCAGTTCATTGTATCGAGATTGTCGCAGCCT
CCCGTAACATATATTCTCCCATCAAGAGTACAAGCAGATGGAAACACACGGCCCACCCGC
ATGCTTGGAGCTTCATGCCATGTGTGAGAACGAGAGTTCATAATCATAACGCTAGACAAA
GCGTTATGATTATGAACTCTCGTTCTCACACATGGCATGAAGCTCCAAGCATGCGGGTGG
GCCGTGAGTTTCCATCTGCTTGTACTCTTGATGGGAGAAT

Last Updated on 06.12.2018 16:11