Sequences from confirmation amplicon


Confirmation sequences for line 187G01 and hit At5g57190 are shown.
Line ID 187G01
Locus At5g57190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23175168
Primers used for PCR 13hw - 8474, show primer details
   
Sequence Name 41-K112667-0022-187-G01C-13hw
GenBank Accession LN504136 [GenBank]
Sequencing primer gene specific primer, 13hw: 5'-TTACTGAGTTCCAAATTGGCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112667-0022-187-G01C-13hw
ACTCTGTACTATCATAATCCTTGGATCTTTACGAGTAGAGAATTACAAAATGAATATTTG
GCAGACTTACGTATCTGAGATTTCAGTTCTGAAAGTCTGTTCACCGAAAGAAACACAAGC
GAGCCACTTGTCCTTAAACTTCATTTCCGCCTGAAGAAAGAAACAAGCCAGAACCAAGAC
TAAAACATAACATCACTACTCACAAACGTAAGAACGAAAAAAAAAAAAAAAAACCTTAAA
ACTTCCCCCATTGAAGGGGAAAACCCCATGGCCGAAAAATCCCCGGGGGAAAACGTTTCT
TTGTACCCCCTTCCCGGCCGGTTTTCAAAAACTCCAAAAATAACACGGGGAAAGGCAAAA
TTTTGGAACCTAATGTCGAACCCTGAACCGTCCCAATCCCTAACCCCCCCTGGGTTTCCC
TTTTCATTGGAAAAGGGCTCCGGGCCGGGAAAATCTCCGGGGATCACCATGGAGTAGAAG
GGCAAATTGGGAAAAAAAAAAAGAGTATTTCCCATTTTTTTTTCATTTAAAAATGGGAAA
TTCCCCCACCGTTTTAAAAA
   
Sequence Name 09-K112666-0022-187-G01C-8409
GenBank Accession LN490251 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K112666-0022-187-G01C-8409
CATTGAAACGGAACTCAAGGGAGGCTAAGGAATCGAGACGGTCAAGGCTACGACATAAGC
TACAAAAGTTTCGCATTCACCGTCGTCATCTTCGTAGTTCTCGAAACTCGGCAGGAATGG
TGATACAACGAACTGTTTCCGCCGAGGATTTCTCCGGCATTGCGCTTCTCACTCTCATTG
GCGTACGTCTTACGTTTTTTTTTTTTTTTTTCCTTTCTACCTTTGGGAAATAGGGATTTT
TTTTTTTATTTTGGGTTCGGGTTTTTTTTTTTTTTCGGGGGAAAATAAATTTAAGGGAAA
GGGGGCCCCTTGGTTTTTTTTTGGGGAAAAAAATTTTCAAAATGAAATTCCAAAAACCTT
AATTCGCCCAAATTTTCTTTTTGAATTTCCCCCCCCGAAAAAACCCGGGGTTTTTGGATA
GAAACAAATTTTTTTTTCAAAAAATTTGGCATACCCCAAAACCCTTTTGGGAATTCATAA
AA

Last Updated on 06.12.2018 16:11