Genotyping and primer details


Genotyping and primer details for 187G01 - At5g57190
Line ID 187G01
Locus At5g57190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23175168 of line 187G01 Chr5:23175168
   
Insertion allele amplicon 8474 - 13hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13hw: 5'-TTACTGAGTTCCAAATTGGCTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23174883
   
wt amplicon (over InsPos) 37si - 13hw
wt amplicon start Chr5:23174883
wt amplicon end Chr5:23175978
wt amplicon size 1096 bp
Locus specific primer 13hw: 5'-TTACTGAGTTCCAAATTGGCTTCT-3'
2nd locus specific primer 37si: 5'-CCTCTTGATATAAAGGGATCACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11