Sequences from confirmation amplicon


Confirmation sequences for line 188F08 and hit At1g08900 are shown.
Line ID 188F08
Locus At1g08900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2854491
Primers used for PCR 03qk - 8474, show primer details
   
Sequence Name 50-K115459-0022-188-F08C-03qk
GenBank Accession LN507272 [GenBank]
Sequencing primer gene specific primer, 03qk: 5'-CATATGCACATTCCGATAGAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115459-0022-188-F08C-03qk
TATGATATCAAATCTGAAATATTATTTAGCTAAGGAAATCAGTAATGAATTTTATAGTGA
AATGATAACCATAAGAAGTGGTCGGCGTCCCCATCGGTCAACGGTTAACATGACAACTAT
AGATTGCGGTATCTTTACAAAAGAAAAAAGTTGTGATTAAATCATAAAGATGAAATATTC
AAAGACAAATAGATGATAAAGAGTAACAAAGAGAATAATGTACCAGAATCACTGCGAGGA
TGGTTGTTCCTATGTCGCTTGGAAAACCTAATTACACAAAACAGTATAATTAAAAATCAC
AACACTGAGTATTAATATGAAGTATTTACAATTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGATTATT
   
Sequence Name 01-K115458-0022-188-F08C-8409
GenBank Accession LN486702 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115458-0022-188-F08C-8409
TTGAATATATCCTGAATTGTAAATACTTCATATTAATACTCAGTGTTGTGATTTTTAATT
ATACTGTTTTGTGTAATTAGGTTTTCCAAGCGACATAGGAACAACCATCCTCGCAGTGAT
TCTGGTACATTATTCTCTTTGTTACTCTTTATCATCTATTTGTCTTTGAATATTTCATCT
TTATGATTTAATCACAACTTTTTTCTTTTGTAAAGATACCGCAATCTATAGTTGTCATGT
TAACCGTTGACCGATGGGGACGCCGACCACTTCTTATGGTTATCATTTCACTATAAAATT
CATTACTGATTTCCTTAGCTAAATAATATTTTTCAGATTTGATTATCATTATTGTTTTTT
ATTTTGCAGATTTCTTCTATCGGATGNTGCATATG

Last Updated on 06.12.2018 16:11