SimpleSearch - Line and FST details
Line specific information
| Line ID | 188F08 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N418020) |
| Segregation Analysis | 50:30:22 |
| Confirmed for Hit | At1g08900 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 96389, 96403, 96413, 96435, 96446, 96458, 96459, 96460, 96462, 96464, 96465, 96467 |
| Note |
Gene hit At1g75480
| Sequence (A. th genome BLAST matches underlined) | >62-K014620-022-188-F08-8409 TGTATGATTTCCCGAACATGAAGACCTGTGGATCATCTGCGATTCCCACTCTGAGGATGC AATCCAGGTGACCCAGCATGCTGCTATGGGATCCTCCCTATAGTGAGACGGTTGTACGTG CTAGGAAATAAATTGTTAAACTATTTTACGCTATGGGATCCTCCCTATACCGAGCGATGC AATCTAGACGCACACCCTATTGCCAGTACTATATCCGCGCACCATATAATCATGATCCTA CATAAAATATAATCAAAGGTGATAACTATGCTATGGATATGGGATTCACTAATGGATGAG GGGAAACGCCTGCCCTTTTTATTAGCACGGCACACATTACGTAACATCTCATGAAAAAGC TTTTGAAGGTCTTTTATGGCAACATCTCGATCATGAAGTATGTATCTGCC |
| GenBank Accession | AL759581 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:28334660 - go to primer design |
| BLAST e Value | 3e-17 |
| Hit Clone Code (BAC ID) | F1B16 |
| Hit Gene Code | At1g75480 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At1g66800
| Sequence (A. th genome BLAST matches underlined) | >62-K014624-022-188-F08-8409 TTGATCCATGTAGAATTTCCCGGGACATGAAGCCTTTACAATTGAATATATCCATACTAC CCAAAGACTGTTTAAATCTGAGAGAGTGGTAAAATTTAATCTTTCTCTAAAGAAACACTC TTTCTAAAACGAAAATGGTCAAATTGCATGTATTTGACCTTCCAGAACCTATGGGATCCT CCCTATNAGTGAGANCACNGCNCACCCNTTGACCATTACATTTTTGTGAATTATACACTC ATGATACCTCATGAAATGAAACCAGCGGTCCATAGCTATCCTTCACTATGGGGTCCGACG TATACGCAGACCACTTCTTATGGCTATCATTTCACTATAAAATTCATTACTGATTTCCTT AGCTAAATAATATTTTTCACATTCGATTATCATTATTGGTCGTTATGGGGCACACTCCTT CTATCGGAATGTGCATATGCACCGCCTTTATTCGGCCTTCATACCCCCCTCCAGGTTAAC GCAACCCTAAATACCCAAATCG |
| GenBank Accession | AL759582 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:24926356 - go to primer design |
| BLAST e Value | 8e-61 |
| Hit Clone Code (BAC ID) | F4N21 |
| Hit Gene Code | At1g66800 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | NAD(P)-binding Rossmann-fold superfamily protein |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | unknown |
Gene hit At1g08900
| Sequence (A. th genome BLAST matches underlined) | >62-K014624-022-188-F08-8409 TTGATCCATGTAGAATTTCCCGGGACATGAAGCCTTTACAATTGAATATATCCATACTAC CCAAAGACTGTTTAAATCTGAGAGAGTGGTAAAATTTAATCTTTCTCTAAAGAAACACTC TTTCTAAAACGAAAATGGTCAAATTGCATGTATTTGACCTTCCAGAACCTATGGGATCCT CCCTATNAGTGAGANCACNGCNCACCCNTTGACCATTACATTTTTGTGAATTATACACTC ATGATACCTCATGAAATGAAACCAGCGGTCCATAGCTATCCTTCACTATGGGGTCCGACG TATACGCAGACCACTTCTTATGGCTATCATTTCACTATAAAATTCATTACTGATTTCCTT AGCTAAATAATATTTTTCACATTCGATTATCATTATTGGTCGTTATGGGGCACACTCCTT CTATCGGAATGTGCATATGCACCGCCTTTATTCGGCCTTCATACCCCCCTCCAGGTTAAC GCAACCCTAAATACCCAAATCG |
| GenBank Accession | AL759582 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr1:2854457 - go to primer design |
| BLAST e Value | 2e-40 |
| Hit Clone Code (BAC ID) | F7G19 |
| Hit Gene Code | At1g08900 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Major facilitator superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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