Sequences from confirmation amplicon


Confirmation sequences for line 189E05 and hit At4g21830 are shown.
Line ID 189E05
Locus At4g21830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11584995
Primers used for PCR 14qv - 8474, show primer details
   
Sequence Name 66-K115779-0022-189-E05C-14qv
GenBank Accession LN511679 [GenBank]
Sequencing primer gene specific primer, 14qv: 5'-ACTTTGAATCACCATTAAACCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K115779-0022-189-E05C-14qv
CAACAAAGGTCAAAGCTATTTCTTTTTTTTGCTAAGAAAAACTAATTTCTTCCTCTTCGA
CCTCTCAAGCATCATTAGATAAACAAATCCTATTCCCGTCATGGCAGCAATGACCGCGGC
AGCAGTTCCAGCAACTGGATCTTTCCAGAAACAAGACGAGGAGTGGCGTGCGGTTCTGTC
TCCTGAGCAGTTTAGGGTTCTCAGACTAAAAGGCACAGAGTAAGAAGTCCTCTAGGGTTT
TTATTTTTATTTTTCTGTTTACTTGAGCAGCAAGAAGTTTACCTGAACATGTTGTTAATG
GTTAATTTGGTGATTTAGTAAACGAGGCAAAGGAGAGTTTACAAAGAAGTTCGAGGAAGG
GAAATCTAATATATATTCAATATATACTCATTTGCTGATCCATATTTACATTTTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAA
ATTATA
   
Sequence Name 18-K115778-0022-189-E05C-8409
GenBank Accession LN494445 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115778-0022-189-E05C-8409
TTACATTGAAAATGTAAATATGGATCAGCAAATGAGTATATATTGAATATATATTAGATT
TCCCTTCCTCGAACTTCTTTGTAAACTCTCCTTTGCCTCGTTTACTAAATCACCAAATTA
ACCATTAACAACATGTTCAGGTAAACTTCTTGCTGCTCAAGTAAACAGAAAAATAAAAAT
AAAAACCCTAGAGGACTTCTTACTCTGTGCCTTTTAGTCTGAGAACCCTAAACTGCTCAG
GAGACAGAACCGCACGCCACTCCTCGTCTTGTTTCTGGAAAGATCCAGTTGCTGGAACTG
CTGCCGCGGTCATTGCTGCCATGACGGGAATAGGATTTGTTTATCTAATGATGCTTGAGA
GGTCGAAGAGGAAGAAATTAGTTTTTCTTAGCAAAAAAAAGAAATTAGCTTTTGACCTTT
TGTTTGAAGTTTTGTACTAAAGACCTGGTTTAATGGTGATTCAAA

Last Updated on 06.12.2018 16:11