Sequences from confirmation amplicon


Confirmation sequences for line 190A05 and hit At2g48100 are shown.
Line ID 190A05
Locus At2g48100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19672097
Primers used for PCR BL75 - 8474, show primer details
   
Sequence Name 30-K105138-0022-190-A05C-BL75
GenBank Accession LN499715 [GenBank]
Sequencing primer gene specific primer, BL75: 5'-CTGTGTCTCTGAGGTGAAGCATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K105138-0022-190-A05C-BL75
CAAAACGGGTCAGTTAGTTACCTCAACAAATGGCTAGGGTATTCAAGCTTCAAGCAACTC
ATATCATGCCTAAGGTCATGACCAACAAGAAGAAGCCTTCCAGCCCCATCATTCTGTCCA
CCGCACAAGAACGAAAAAACTCTCTCTCGTACATGCTCAAGTGGCATACCATCCTTCAAA
TCTTCCTTAGTCAATCCAGTTATCTCGTGCCTGAACATGAGGCGGGGTGAGATTTAAAAG
GAAGAATTATAAAACAAAACAAGCACAAATGGTCCACAAGCAGAACCTGTAATCGGTGAC
AGGGAAACCAATATATCCATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 14-K105139-0022-190-A05C-8409
GenBank Accession LN492502 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K105139-0022-190-A05C-8409
CATTTACAATTGAAATATCCTGCATGGATATATTGGTTTCCCTGTCACCGATTACAGGTT
CTGCTTGTGGACCATTTGTGCTTGTTTTGTTTTATAATTCTTCCTTTTAAATCTCACCCC
GCCTCATGTTCAGGCACGAGATAACTGGATTGACTAAGGAAGATTTGAAGGATGGTATGC
CACTTGAGCATGTACGAGAGAGAGTTTTTTCGTTCTTGTGCGGTGGACAGAATGATGGGG
CTGGAAGGCTTCTTCTTGTTGGTCATGACCTTAGGCATGATATGAGTTGCTTGAAGCTTG
AATACCCTAGCCATTTGTTGAGGTAACTAACTGACCCGTTTTTGTTGACTCTTTGCTTGA
AATCTAATGTAATTGCTATGCTTCACCTCNAAGACACA

Last Updated on 06.12.2018 16:11