Sequences from confirmation amplicon


Confirmation sequences for line 190F11 and hit At4g02080 are shown.
Line ID 190F11
Locus At4g02080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:921560
Primers used for PCR TB03 - 8474, show primer details
   
Sequence Name 17-K020131-022-190-F11C-TB03
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, TB03: 5'-CCAGTTAACCATTGTTCGCTTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K020131-022-190-F11C-TB03
GCTAAACAAACCAAAAATTCCCATTCCTGATTATGAAGGATTTCCATTGGAAACACCAAA
ATCCCCCAAATTTTTAAAAAATTTTTGAACTTACCTTACCTACCCGGATTGGCGCCAAAG
AAAAATTTTTTTTTGTCCCCAAAAGGCCTTTGGAACTTTAGCTAATGGAAATATATTAAA
GGGGGTATTGGGGCCCCCCAAGGGGGTGCCCTTTACGGAAAAAAAAAAAACATCCCTGTG
ACTCCCCCTTGTGATTTAAGAAACCCCCCCGCTTGGCGGGTTCCCGTTCTACCTTACTTT
   
Sequence Name 18-K020131-022-190-F11C-8409
GenBank Accession LN494121 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K020131-022-190-F11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACATGACTAACTCAG
ATCCTCCAACCACAAAGCTCAAATATTATCCGATCGAGAGAGAGAGAGAGAGAGAGATCG
TCGGGTTTCTAATTTAGCTTTGAAGACGACGAGAGAGGCGTTTCTGAATTGAAAAGTAAA
GTAGAAACGGAAAACGGCAAGCAGTGCGGATTCCTAAATCACAAACGGGAGTCACAACGA
TGCTTCTGTCTTCTACGTCAAAGGCGAGCCCATTAGAGGACCCAATAAGCCCATTTATAT
ATTTACATTAGCTCAAGTTCCAAATGGCCTTTTAGGACAAGAAATAAATTTTCCTTTGCG
ACAATACGGGTGTGTAGTGTAAGATCTAAAATATTTTTAAAATTTTGTCGATTTTTGTGT
TTTCAATTGAAATACTTCATAATCATGAATCGGAATTTATCGTTTGTTTATAACGTCATA
GCATAATTAGAACACATTTTAAATTTGATTCTATTAATTTGTGTAACATTACACAAAACA
ATTTTACGACTGATTACACAACATTTAGGGTTAACTGGTATATCAGATAAATAAATGAAT
TGCAGAATAAGC

Last Updated on 06.12.2018 16:11