Sequences from confirmation amplicon


Confirmation sequences for line 191C09 and hit At5g01950 are shown.
Line ID 191C09
Locus At5g01950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:368357
Primers used for PCR NH55 - 8474, show primer details
   
Sequence Name 24-K102590-0022-191-C09C-8409
GenBank Accession LN496945 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K102590-0022-191-C09C-8409
CAGTACAAAGTTGAGCTGCTAAAAATCACCTCTGTAAACCTTTAACCATCATGATGTAAC
AAATCATGGGAACAGTGGCAACATCGTGGCAATGACTCACACATGAAAGATGTTAGTAAG
ATTGGAAAGCTCAACTGGAATCTGGCCGGTCAACGAATTATTGTTGAAGTGACTGTACAA
GGCCAAAAGAAAAATATATGAGATGATATTGAAAAAGCTGCGTCTCAGTTTGATATCACT
GTCTGGCTTGATGTTCAGACTCACAGATGTTTCACTTTTTTCAAGTTGGAAAAGGACTTT
GGTATTGGTCCTGTGATGTTATTCTCATCTATCTGGAACCTGTTCAAGTTCGAAAGATAC
CCGAGTTCGCTTGGTAAAGTCCCTGAAAGTTTGTTTCCATTCAGCAGCCTGCCCACAGGT
TACAGAATCAGAAGATAAAGAAAAGGAACACACATGCTTATAAAAAATTGTTCCATCTAG
AGCAATGATTATTTATCAGGTAGGTCCAACTTACAAGAGTACCAGGGATGATATCTGTCC
TATCTCGTTTGGAATTGAACCACTTATATTATTCCACATGAAATCTCTTCATAGATGCCA
CACGAAAGAAATATTAGACAGTCAGTAGTGTAACCAAAATATAGGGCGTTCCAAAATAAA
CACTTTGCAAAGCTTTACATGTTGAAACTTACAATATTTCGAGATGAGCTAATTTTTGGA
GCTCTGGTGACAAAGTCCCGGAGAGATTCATATTCATCAGCAGGCTGCTTGGCAATAAAA
GGAAGGATCAGGGAAGGAATATCTGGTCATGTAAGCAACTGTTCCGAGAAAAGAGAAAAA
AAAAGACAACTGAACCAGCTTCTCCAA
   
Sequence Name 24-K102591-0022-191-C09C-NH55
GenBank Accession LN496946 [GenBank]
Sequencing primer gene specific primer, NH55: 5'-AAAAGGCTTTACTTTTGGGAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K102591-0022-191-C09C-NH55
TTTTTTTCCTTTTCCGGAACGTTGCTTACATGACCAGATATTCCTTCCCTGACCCTTACT
TTTATTGCCAAGCAGCCTGCTGATGAATATGAATCTCTCCGGGACTTTGTCACCAGAGCT
CCAAAAATTAGCTCATCTCGAAATATTGTAAGTTTCAACATGTAAAGCTTTGCAAAGTGT
TTATTTTGGAACGCCCTATATTTTGGTTACACTACTGACTGTCTAATATTTCTTTCGGGT
GGCATCTATGAAGAGATTTCAGGTGGAATAATATAAGTGGTTCAATTCCAAACGAGATAG
GACAGATATCATCCCTGGTACTCTTGTAAGTTGGACCTACCTGATAAATAATCATTGCTC
TAGATGGAACAATTTTTTATAAGCAGGTGGGTTCCTTTTCTTTATCTTCGGATTCGGTAA
CCTGTGGGCAGGCTGCTGAATGGAAACAAACTTTCAGGGACTTTACCAAGCGAACTCGGG
TATCTTTCGAACTTGAACAGGTTCCAGATAGATGAGAATAACATCACAGGACCAATACCA
AAGTCCTTTTCCAACTTGAAAAAAGTGAAACATCTGTGAGTCTGAACATCAAGCCAGACA
GTGATATCAAACTGAGACGCAGCTTTTTCAATATCATCTCATATATTTTTCTTTTGGCCT
TGTACAGTCACTTCAACAATAATTCGTTGACCGGCCAGATTCCAGTTGAGCTTTCCAATC
TTACTAACATCTTTCATGTGTGAGTCATTGCCACGATGTTGCCACTGTTCCCATGATTTG
TTACATCATGATGGTTAAAGGTTTACAGAGGTGATTTTTAGCAGCTCAACTTTGTACTGT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT

Last Updated on 06.12.2018 16:11