SimpleSearch - Line and FST details
Line specific information
| Line ID | 191C09 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N418273) |
| Segregation Analysis | 50:50:41 |
| Confirmed for Hit | At5g01950 At3g48770 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 3174, 95571 |
Gene hit MHM17
| Sequence (A. th genome BLAST matches underlined) | >67-K014642-022-191-C09-8409 TTGATCCATGTAGAATTTCCCGGACATGAAGCACTTTACAATTGGAATAAGGTACAAGTT AATAGTAATCTAACTCTAACCTAATAATAATCACACAAAATGAAAGTTTAAGCTTAATTT TGTATGAATTTTATATGAATTTTTTAATCTTTTAAAAAATAGTAAAATAACTCCTAATAT TAATAAAATAAACAAATTAGCGACCAAAGAGAGTTTATGAACTGATTCTCTTGAAGCAAG TGTATCTTCAAAAGAGCTGATACTCTCCTCACCAAAGCGATAGCCCAACATATACCGCTA TGGGATCCTGCCTATAGTGAGA |
| GenBank Accession | AL759829 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:23063521 - go to primer design |
| BLAST e Value | 1e-47 |
| Hit Clone Code (BAC ID) | MHM17 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit At5g01950
| Sequence (A. th genome BLAST matches underlined) | >67-K014644-022-191-C09-8409 NTGATCCATGTANGCATTTCCCGGACATGAAGCCTTTACAATTGGGTGGCGGCTTATAAC ATAGATCGCATGATATGTACCGAGATGATCCCCACGGGCCTCTTTTCACCTCCTACTGCC GAGGAGAATGGGCGTGTCATGGCATGGGCTGACATGGATCCTCCTATGGCATGCTGCCTG TAGTAAGATCGTCTTGCGCACGAAATACCGAAATGTCCCCATGGGCATCTATTCCACCCG CCTATGGGATACTGCCTATAATGCGCTCCTCACCATAGATGATAGCGTGCGTATACCCCT ATGGACATCCTCCCTATACAGTGTTTTCAAGTTGGAAAAGGACTTTGGTATTGGTCCTGT GATGTTATTCTCATCTATCTGGAACCTGTTCAAGTTCNAAAGATACCCGAGTTCGCTTGG TAAAGTCCCTGAAAGCTTGTTTCCATTCAGCAGCCTGCCCACAGGTTACAGAATCCGAAG ATAAAGAACAGGAACACACATGCTTATAACACATTGTTCCACCTATGGGATCCTCCCTA |
| GenBank Accession | AL759830 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:368331 - go to primer design |
| BLAST e Value | 3e-94 |
| Hit Clone Code (BAC ID) | T20L15 |
| Hit Gene Code | At5g01950 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Leucine-rich repeat protein kinase family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At3g48770
| Sequence (A. th genome BLAST matches underlined) | >59-K114351-0022-191-C09C-8409 GCTATAATAACGCTGCGGACATCTACATTTTGAATTCCGCTTTTGACAGAACAGAATATG ACAACGTTTTGAACTTCTTGGGTCTGAAGCAAGTAAGCAACGAATGGTATGTAAAATGCA TTCAGGGCTGTGATCTCGTGACAAGTGTGTCAGAAGCTACGTATGTGGAGGTTCTACTCT TCATCGCTGAGAATTGGCAGTGCAGGTTTCAGAATACAAATATGGGGAAAGTTCCCCTTA TCAAGTATGTTGTCCAAAAGGGAGTGAGATCTCTCAGCAGTTTGGGTGTTTTCAGTCCTA GGACATTATGCTTGTCCACAGAGAAGAACCAGGCAAGGTTACGTGAATGGAACGATGAGT TCAGATGTATGTCGAACTTCGTTTACATGCCACCAACAATCCCGGACAGCGTTGA |
| GenBank Accession | LN713398 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:18082984 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | T21J18 |
| Hit Gene Code | At3g48770 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | ATP/DNA binding protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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