Sequences from confirmation amplicon


Confirmation sequences for line 191C11 and hit At3g61310 are shown.
Line ID 191C11
Locus At3g61310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22691532
Primers used for PCR 07nt - 8474, show primer details
   
Sequence Name 70-K114626-0022-191-C11C-07nt
GenBank Accession LN512436 [GenBank]
Sequencing primer gene specific primer, 07nt: 5'-GTTGGCATTGTCTCCTTCTATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K114626-0022-191-C11C-07nt
TCCTGGCTCCGGCAGAACAACGGCTATCTTCCATTGGTAATATCCCTAATCTCTAACTTA
TGAGTTTTATTTTCCGACAAGGACATTGGCTCTGAATATGGTGGGAAGTTTTGAGCTTAA
TTGATGATATAGTTTTTCAAATGAGGACATTGGTGGGAAGTTTATTATAAGCTAATTCGA
ACTGTTAAGGATTAATTAAAGCTGTGGAATTTTATAATGTCAAATTGTTGAATTCAATAT
TTATTCTTTTATTATTCCTTGGCCTTTCTCTTAAACTTTGTTATTGCTCTGTTTCTCCCT
GATTACAATTAGCTGTGATTTTTCTGAAGACATTTGATTCTTTTTTGTAAGGTGAAATGA
TGCCTTCATCAACTGGGATGAGCTTCACACCGCAGGTAATCGTAGTTTCCATTGGTGAAG
TATGTTTCTTCTGTCTCCTCTTGAATAAATCTGTTCTTTTTTTTCTTTGTAATCTTGCCT
CTNGTTCTTGAGAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTC
   
Sequence Name 70-K114625-0022-191-C11C-8409
GenBank Accession LN512435 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K114625-0022-191-C11C-8409
TTGAATATTCTCAAGAACTAGAGGCAAGATTACAAAGAAAAAAAAGAACAGATTTATTCA
AGAGGAGACAGAAGAAACATACTTCACCAATGGAAACTACGATTACATGCGGTGTGAAGC
TCATCCCAGTTGATGAAGGCATCATTTCACCTTACAAAAAAGAATCAAATGTCTTCAGAA
AAATCACAGCTAATTGTAATCAGGGAGAAACAGAGCAATAACAAAGTTTAAGAGAAAGGC
CAAGGAATAATAAAAGAATAAATATTGAATTCAACAATTTGACATTATAAAATTCCACAG
CTTTAATTAATCCTTAACAGTTCGAATTAGCTTATAATAAACTTCCCACCAATGTCCTCA
TTTGAAAAACTATATCATCAATTAAGCTCAAAACTTCCCACCATATTCAGAGCCAATGTC
CTTGTCGGAAAATAAAACTCATAAGTTAGAGATTAGGGATATTACCAATGGAAGATAGCC
GTTGCTTCTTGCCGGAGCCAGGAGGTCTTCCACGGCCACGTTTGTTAGAGTTCGGGGAAA
CGTTGGAGATAGAAG

Last Updated on 06.12.2018 16:11