Sequences from confirmation amplicon


Confirmation sequences for line 191D02 and hit At2g46340 are shown.
Line ID 191D02
Locus At2g46340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19025482
Primers used for PCR 13fh - 8474, show primer details
   
Sequence Name 21-K110985-0022-191-D02C-13fh
GenBank Accession LN495714 [GenBank]
Sequencing primer gene specific primer, 13fh: 5'-AAGCTAGATGTCAGTAGGCGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K110985-0022-191-D02C-13fh
CAAATACTGACAGTTATATACTGCAGACTGGAAAATACTAGCTCTGGGATTCAAAGTTCA
GACTAGAGTAAGAAATTTCATGAAAATGAAAGAATATAAACTACCCAAAGCCCACATAAG
CAGGATATGACAGTACATAGTGTTCAAATGCAAGGAAATGTTACATCCTTCTTGGTATTA
AAGCAAACTAAAAATCTATCCACAACAATAAAACTGGGTATTCGTCCATACCTCAAACAG
AAGAACACCAAGGGCATAAATATTTGATTTTTCTCCAATATCTTCTCCATTTATCTATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 05-K110984-0022-191-D02C-8409
GenBank Accession LN488412 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K110984-0022-191-D02C-8409
TCATTGAAAGATAATGGAGAAGATATTGGAGAAAAATCAAATACTTATGCCCTTGGTGTT
CTTCTGTTTGAGGTATGGACGAATACCCAGTTTTATTGTTGTGGATAGATTTTTAGTTTG
CTTTAATACCAAGAAGGATGTAACATTTCCTTGCATTTGAACACTATGTACTGTCATATC
CTGCTTATGTGGGCTTTGGGTAGTTTATATTCTTTCATTTTCATGAAATTTCTTACTCTA
GTCTGAACTTTGAATCCCAGAGCTAGTATTTTCCAGTCTGCAGTATATAACTTGTCAGTA
TTTTTGTTTACTTGTTTGATACTTTCATGTGAAGACGCCTACTGACATCTAGCT

Last Updated on 06.12.2018 16:11