Sequences from confirmation amplicon


Confirmation sequences for line 191D09 and hit At5g04630 are shown.
Line ID 191D09
Locus At5g04630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1331150
Primers used for PCR MS62 - 8474, show primer details
   
Sequence Name 37-K017177-022-191-D09C-MS62
GenBank Accession LN502431 [GenBank]
Sequencing primer gene specific primer, MS62: 5'-CCGGGAAGATAAAACTCAACATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K017177-022-191-D09C-MS62
AGGAATTCGCAACCCGCGAGATTAGTCGGCTCCGTAACGCCGTGAGTTAACGTGAAATAC
GTCGGTGGATGTCTCCGGAGAAGCTCCTTAACAAAAGCTTGTAAAAAGACCATTTTATTC
AAATCTTTCTCCTCAACCGTTCGATCATCTCCCACCGTTGATTTAATCTCATCGTACAGC
CGAGATTGAATCTTCGGATTCGATATCAGCTCCGCGATTCCCCACTCTATCGCCGTCCCC
GTCGTATCCGTACCGGCGTTGAGAAACTCTGAACACAGCGTCACGAGATCTTCATCTGAC
GGTGACGTTTCACGACCATCAACTCTGAGATCGAATAAAGTATCAAGATACGCGAAAGAC
GACGCCGTTTTATCGGAGCCGAGATTTCGAATCGCTAATCGTCGTTTCTCGATGAATCCA
ACGACGAATTGGACAAGTTTCCGGCGAAGTTCCAGAGAATTTTTCCTCTCCTTGAAGTAA
AACGGAGTTAGAATCGGAAGATAGTCGTGAATTCGTGGATCAACGGCGGTTAAAATCTCC
GTCATCATCTGATCCATCTTCTCAATCGATTCTTCTTCCATTTTAACTCCGAAACACATA
TCTAACAAGATACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTTCAATTCAA
AAA
   
Sequence Name 41-K017177-022-191-D09C-8409
GenBank Accession LN503892 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K017177-022-191-D09C-8409
GATTTCCGGATCTGAAGCCATTTACAATTGTATCTTGTTAGATATGTGTTTCGGAGTTAA
AATGGAAGAAGAATCGATTGAGAAGATGGATCAGATGATGACGGAGATTTTAACCGCCGT
TGATCCACGAATTCACGACTATCTTCCGATTCTAACTCCGTTTTACTTCAAGGAGAGGAA
AAATTCTCTGGAACTTCGCCGGAAACTTGTCCAATTCGTCGTTGGATTCATCGAGAAACG
ACGATTAGCGATTCGAAATCTCGGCTCCGATAAAACGGCGTCGTCTTTCGCGTATCTTGA
TACTTTATTCGATCTCAGAGTTGATGGTCGTGAAACGTCACCGTCAGATGAAGATCTCGT
GACGCTGTGTTCAGAGTTTCTCAACGCCGGTACGGATACGACGGGGACGGCGATAGAGTG
GGGAATCGCGGAGCTGATATCGAATCCGAAGATTCAATCTCGGCTGTACGATGAGATTAA
ATCAACGGTGGGAGATGATCGAACGGTTGAGGAGAAAGATTTGAATAAAATGGTCTTTTT
ACAAGCTTTTGTTAAGGAGCTTCTCCGGAGACATCCACCGACGTATTTCACGTTAACTCA
CGGCGTTACGGAGCCGACTAATCTCGCGGGATACGATATTCCCGTCGGGGCTAATGTTGA
GT

Last Updated on 06.12.2018 16:11