Sequences from confirmation amplicon


Confirmation sequences for line 191F06 and hit At1g80900 are shown.
Line ID 191F06
Locus At1g80900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30399068
Primers used for PCR 04qk - 8474, show primer details
   
Sequence Name 56-K115459-0022-191-F06C-04qk
GenBank Accession LN509195 [GenBank]
Sequencing primer gene specific primer, 04qk: 5'-GCAGAGACTAAAGGCAAGTTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K115459-0022-191-F06C-04qk
AGGAGTTTAGACATGTGCTTCAGAACTCATCCCCTGATTATTTACCTTTCGAGTTCAGGG
CTCTTGAAGTTGCACTTGAAGCTGCTTGTACCTTCCTTGATTCCCAGGTCAGTGATTGAT
TCACAACTTATTGTTATTTAGTATGCTCAGCATTGAGAATAGATTTTCCCTTTGTAGGCT
TCAGAGTTAGAGATTGAGGCATACCCATTATTGGATGAGCTTACCTCAAAGATCAGTACT
CTAAACTTGGAGCGTGCTCGTAGACTAAAGAGCAGACTTGTAGCATTGACGAGACGAGTT
CAGAAGGTTCGGGATGAGAAGAAATGTATCAATCTTTATATCCGACAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
C
   
Sequence Name 07-K115458-0022-191-F06C-8409
GenBank Accession LN489407 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115458-0022-191-F06C-8409
TTACATTGATAATCCTGTCGGATATAAGATTGATACATTTCTTCTCATCCCGAACCTTCT
GAACTCGTCTCGTCAATGCTACAAGTCTGCTCTTTAGTCTACGAGCACGCTCCAAGTTTA
GAGTACTGATCTTTGAGGTAAGCTCATCCAATAATGGGTATGCCTCAATCTCTAACTCTG
AAGCCTACAAAGGGAAAATCTATTCTCAATGCTGAGCATACTAAATAACAATAAGTTGTG
AATCAATCACTGACCTGGGAATCAAGGAAGGTACAAGCAGCTTCAAGTGCAACTTCAAGA
GCCCTGAACTCGAAAGGTAAATAATCAGGGGATGAGTTCTGAAGCACATTGTCTAAACTC
CTACTCCTCCTCCTGCTCAATTCGAGACTGTCCTGATTCCAAACCTCAGTAACAGAACTT
GCCTTAGNTCTCTG

Last Updated on 06.12.2018 16:11