Sequences from confirmation amplicon


Confirmation sequences for line 192G12 and hit At5g46990 are shown.
Line ID 192G12
Locus At5g46990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19067845
Primers used for PCR 04xq - 8474, show primer details
   
Sequence Name 48-K119542-0022-192-G12C-04xq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 04xq: 5'-TGAATAGAAATGGTACCAAGGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K119542-0022-192-G12C-04xq
TGTTTTGTTTTTTTTTTTTTTTTTCCCCCC
   
Sequence Name 16-K119541-0022-192-G12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119541-0022-192-G12C-8409
ATTGAATATATCCTGCAAGAGTTGTTCCGAGAGAGTGTGAAATGGGATTCAATGACGATA
ATAAACAGAAATCTCCGGTGACGAAAGAGAACGATGTTCTATTCGACACCGTCGACATTG
CTCAGTCTTTTAATTACTATGCTCATATAAGGCCAGACATCGTATAGTGTCTAGCCTAAC
AGCCCCTCGGTAATGTTTCTTAGTTTGTTATACAACAATGAATTAATGAAAAAATATAAG
GTCAGAGTTTACGTACAACTTTCATCGAGATAGGAATGAATCTATGTTGCACCATTTCAA
ATCATGTCTCCTATTTTCACCTTTTCTAAATTTCTTTGACAAGCTACAACACATATTTAA
TAAAAAGTTTTGTTCTTACCACACTTCTCAATTTTAAAAAATTTCTTTACATATATATAG
TACTTCTTAAGAATAATTCTGAAGAGAGAGGTGAATTCATGAAAGAAGGCCATGTTAAAC
TACGTTTCATTAGCTTATATCTTCCCTGATATAATTTAGACTTTAATATTTAAGTGTTAA
GAAATGACTTCACACAGAGAATTAGTGGAAGGTAAATCGTCTCTTTTCCACATAAAATGC
TTCACATGAAAAGAAAATTTATTTCTTCAGAGAAAAAGAAAGTAAACCTAGCTAGTTGAT
ATTCATTTGATAAAATAAAACTTTCATGCCATATATCAATAGAGATGAACATATACCTTG
TTTTTCATTTCTAGTGATGGTTAAGTCTCTGTTCAGTATACATATNTCTATATGTTCTTT
AGTTTCTGACATATATGAAGATCAAAACTCGATGGTTATGTACTTTTGCAGATCATTGTC
CTTG

Last Updated on 06.12.2018 16:11