Sequences from confirmation amplicon


Confirmation sequences for line 192H02 and hit At5g17700 are shown.
Line ID 192H02
Locus At5g17700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5831094
Primers used for PCR EP16 - 8474, show primer details
   
Sequence Name 32-K114586-0022-192-H02C-EP16
GenBank Accession LN500685 [GenBank]
Sequencing primer gene specific primer, EP16: 5'-TAAAAGTGGTGCTTGTGGTATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114586-0022-192-H02C-EP16
CGGTCAGATTTCGTATTTGTTCTCTGATAGCCAAGCAGTTTCGGATGCAGTAGCTGATCT
CTCCATCGTCCTTAGCATATCTATACTCTTCAACATCATCCAGCCCATTCTATCTGGTTA
CAAAATAAAACCCTTATATTAGACATAAATAAATTATAACATAAACTTTTAGATTTCACT
AATTTGTAACCACTAAATTTGTTTGAACATATATAAACAGGGGTAGCAATTGGAGCAGGG
ATGCAGAGTATGGTAGCATTAGTGAACTTAGCATCTTATTATGCAATTGGTGTTCCTTTA
GGTGTCCTCCTTGTTTACGTTTTCAATTTTGGGATTAAGGTACATCATATATATGAAAAC
TGGTATCCAGTACTTCATTATGATCAATCATTAGAACTTTTAGAATTTTAACTTGAATAA
ATGATATTGTATGCAGGGACTCTGGTCCGGAATGTTGGCTGGAGTTGGTATCCAAACGTT
GATATTGTGTTATGTTATTTACAAAACTGACTGGGAATTGGAGGTAATTTACTTTTAATA
TATAACTCANAATTTTTATTAGAAAATTCGTCATATTAAATGTATAATTTTATCTAATTA
CAGGTTAAGAAGACAAACGAGCGTATGAAAACGTGGACTCTAAATTTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
ANAAATGTAGATTCCGC
   
Sequence Name 28-K114585-0022-192-H02C-8409
GenBank Accession LN498958 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114585-0022-192-H02C-8409
TTTCAACGCTCGTTTGTCTTCTTAACCTGTAATTAGATAAAATTATACATTTAATATGAC
GAATTTTCTAATAAAAATTTTGAGTTATATATTAAAAGTAAATTACCTCCAATTCCCAGT
CAGTTTTGTAAATAACATAACACAATATCAACGTTTGGATACCAACTCCAGCCAACATTC
CGGACCAGAGTCCCTGCATACAATATCATTTATTCAAGTTAAAATTCTAAAAGTTCTAAT
GATTGATCATAATGAAGTACTGGATACCAGTTTTCATATATATGATGTACCTTAATCCCA
AAATTGAAAACGTAAACAAGGAGGACACCTAAAGGAACACCAATTGCATAATAAGATGCT
AAGTTCACTAATGCTACCATACTCTGCATCCCTGCTCCAATTGCTACCCCTGTTTATATA
TGTTCAAACAAATTTAGTGGTTACAAATTAGTGAAATCTAAAAGTTTATGTTATAATTTA
TTTATGTCTAATATAAGGGTTTTATTTTGTAACCAGATAGAATGGGCTGGATGATGTTGA
AGAGTATAGATATGCTAAGGACGATGGAGAGATCAGCTACTGCATCCGAAACTGCTTGGC
TATCAGAGAACAAATACGAAATCTGACCGCCAAACGCTAGGCAAAGAGCGGAACATATCA
CACCGATNACCGCTGATACCACAG

Last Updated on 06.12.2018 16:11