Sequences from confirmation amplicon


Confirmation sequences for line 192H04 and hit At2g28140 are shown.
Line ID 192H04
Locus At2g28140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11995791
Primers used for PCR BL78 - 8474, show primer details
   
Sequence Name 32-K105138-0022-192-H04C-BL78
GenBank Accession LN500579 [GenBank]
Sequencing primer gene specific primer, BL78: 5'-CAACAATGGAGTTAGAGCAAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K105138-0022-192-H04C-BL78
CAAGACGACCAGATATGCAGCTCAAAGATCTTCTCAACAAAGCTATCAAAGAGAAAGATG
AAGCACAAGAGAGATACAAAAGGATTCTTCTTGACCAAAACTTCCTCCTTCAACACCAAA
CGGACGAAGAGCAAGATCCAAACATCAATGGGTTTCACTCATCGGATGGTGAAGAAAGCA
TTGTCTCATCTTTTGAACCGCCGGTGGAAATTGAGTTAGATTTTCCGGAGATGACTTTAC
CGGAGAAAGGGAAGCTTTTAAAGGCGGTGGTGAAGGCAGGGCCTTTGCTTCAGACTCTAC
TTCTAGCTGGACAGCTTCCACAATGGCGTCACCCGCCACCACAACTTGAGTCCTTTGAAA
TTCCACCGGTGATTATTGCTGAAGGATCACCATTGTCACAAGATAGTTGTGGGAATAATC
TAAACAGGAAGAGGGTTCACTGTGATTCCAAAAGAGAGACCAAGTACCCTTTACATGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCC
   
Sequence Name 16-K105139-0022-192-H04C-8409
GenBank Accession LN493426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K105139-0022-192-H04C-8409
CCATTTACAATTGAATATATCCATGTAAAGGGTACTTGGTCTCTCTTTTGGAATCACAGT
GAACCCTCTTCCTGTTTAGATTATTCCCACAACTATCTTGTGACAATGGTGATCCTTCAG
CAATAATCACCGGTGGAATTTCAAAGGACTCAAGTTGTGGTGGCGGGTGACGCCATTGTG
GAAGCTGTCCAGCTAGAAGTAGAGTCTGAAGCAAAGGCCCTGCCTTCACCACCGCCTTTA
AAAGCTTCCCTTTCTCCGGTAAAGTCATCTCCGGAAAATCTAACTCAATTTCCACCGGCG
GTTCAAAAGATGAGACAATGCTTTCTTCACCATCCGATGAGTGAAACCCATTGATGTTTG
GATCTTGCTCTTCGTCCGTTTGGTGTTGAAGGAGGAAGTTTTGGTCAAGAAGAATCCTTT
TGTATCTCTCTTGTGCTTCATCTTTCTCTTTGATAGCTTTGTTGAGAAGATCTTTGAGCT
GCATTATCTGGTCGTCTTTGGCAATGAGTTCTTCGCTGGCCACCATTCTTGTTTGCTCTA
ACTCCCATTGTTG

Last Updated on 06.12.2018 16:11