Sequences from confirmation amplicon


Confirmation sequences for line 195A05 and hit At4g17680 are shown.
Line ID 195A05
Locus At4g17680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9843208
Primers used for PCR 04vi - 8474, show primer details
   
Sequence Name 42-K117932-0022-195-A05C-04vi
GenBank Accession LN504625 [GenBank]
Sequencing primer gene specific primer, 04vi: 5'-TTGTTTGTTGCTTAGATCTGGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117932-0022-195-A05C-04vi
TCATCATCATCAAAATAAAACTTCGACACCGGAAGTCTTCACGGCGAGGCAAATCGGACA
GACTCGCACTGCTCCGACGTCGCATCCATTACAAAGAATCAAATGTTGACACGGCAATGC
CATCACCGTCGCAGATTTCCGCCGGCAAATCCTACAACTTGGTCCGATCAATTCGATCCG
CTCCGGATCCACGTAAACCGATTCAGCGTCCTCTGCTTCTTCCGCGTCTCCGCCGTCTTC
TCCGAACGTACTCTGTTTCGCCGCCGTATCTAGGCGGTTAGCTATGGCTTGTTGGAGATG
AGCATGTAACGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCACGATTATTAT
   
Sequence Name 02-K117931-0022-195-A05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117931-0022-195-A05C-8409
CTCTGGCTTGTTGGAGATTAGCATGTAAATAGGATATATTCAATTGTAAATGGTGTCATG
TCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCGCTTCGGAAATGTAGATGTCCGCAGC

Last Updated on 06.12.2018 16:11