Genotyping and primer details


Genotyping and primer details for 195A05 - At4g17680
Line ID 195A05
Locus At4g17680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9843208 of line 195A05 Chr4:9843208
   
Insertion allele amplicon 8474 - 04vi
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04vi: 5'-TTGTTTGTTGCTTAGATCTGGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9842833
   
wt amplicon (over InsPos) 42vu - 04vi
wt amplicon start Chr4:9842833
wt amplicon end Chr4:9843793
wt amplicon size 961 bp
Locus specific primer 04vi: 5'-TTGTTTGTTGCTTAGATCTGGATG-3'
2nd locus specific primer 42vu: 5'-TTTTTCCTTCAATTCCATTCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11