Sequences from confirmation amplicon


Confirmation sequences for line 195B09 and hit At2g35155 are shown.
Line ID 195B09
Locus At2g35155 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14820957
Primers used for PCR AC78 - 8474, show primer details
   
Sequence Name 08-K104226-0022-195-B09C-AC78
GenBank Accession LN489711 [GenBank]
Sequencing primer gene specific primer, AC78: 5'-ATAAACTTGTTCCTTTGGTGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104226-0022-195-B09C-AC78
CATCTCATCGCACCATACCCCTCCAGGACCCTATTATGAAATGAACACATCGCTTATCAT
ATATGCTCCGATAAAATTTCTGAAAGGCTATGTGTAGAAGGGAATAGAACACAAGATAAC
TCCACCTCAAGGGCAGAAGGAAGGCACTGCATTGGATTTAGCCATTGCCTATGGACTTTC
CTAGCAACAAAGACAAGTATCGCTGGAACATTCGTTAGAACACCACGGCTGATCCGAAAC
CCAACCGCAGTACCAATATGTATGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGGTTATA
   
Sequence Name 24-K104227-0022-195-B09C-8409
GenBank Accession LN496971 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104227-0022-195-B09C-8409
GCCATTTACATTGAATATATACATATTGGTACTGCGGTTGGGTTTCGGATCAGCCGTGGT
GTTCTAACGAATGTTCCAGCGATACTTGTCTTTGTTGCTAGGAAAGTCCATAGGCAATGG
CTAAATCCAATGCAGTGCCTTCCTTCTGCCCTTGAGGTGGAGTTATCTTGTGTTCTATTC
CCTTCTACACATAGCCTTTCAGAAATTTTATCGGAGCATATATGATAAGCGATGTGTTCA
TTTCATAATAGGGTCCTGGAGGGGTATGGTGCGATGTAGATGTTGTGGAATTCCAATACT
ACGGTGCTCCTGCTGCAACACCAAAGGAACAAGTTTA

Last Updated on 06.12.2018 16:11