Sequences from confirmation amplicon


Confirmation sequences for line 196C10 and hit K21P3 are shown.
Line ID 196C10
Locus K21P3 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19968150
Primers used for PCR FA34 - 8474, show primer details
   
Sequence Name 57-K037324-022-196-C10C-8409
GenBank Accession LN509346 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K037324-022-196-C10C-8409
GCCATTTAAATTGAATATATGTAATTGCTGATCAGCCATTTACATCACAGTTTCTTATCC
ATAAATGTTTTGCATATTATTATCACTTTTTCATTATGGATGTTTTCTCTTCATATATTT
TGTGCTAGTAATGGGCGTACAACTTAAACGTTATGGACAACGACGACCACATTATTTGCT
GTTGGAGGAGGAAATACGGCTCATTTATTGAATAGTTGTAATCACTTGCAAAATATTCTA
TTTTTTTATATATATATATCCTATTAGTTGATCCGGTTAGTTTTGGTAGGAACGCACTGA
AGTGTTACAGAGTGGATTTCTCCTGATTCAGATAGAATTGGGCCACCACACTGCCTGATC
CAAGTTTAGAATGTCTTCCGACTAATGTCAGAATATAATCAATAATCAGGCCGCCTGCAT
TTCTAACTACCAGGTAAACCAATTAAAGCAAAACCCA
   
Sequence Name 49-K037324-022-196-C10C-FA34
GenBank Accession LN506758 [GenBank]
Sequencing primer gene specific primer, FA34: 5'-TGGGTTTTGCTTTAATTGGTTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K037324-022-196-C10C-FA34
GCGGCCTGATTATTGATTATATTCTGACATTAGTCGGAAGACATTCTAAACTTGGATCAG
GCAGTGTGGTGGCCCAATTCTATCTGAATCAGGAGAAATCCACTCTGTAACACTTCAGTG
CGTTCCTACCAAAACTAACCGGATCAACTAATAGGATATATATATATAAAAAAATAGAAT
ATTTTGCAAGTGATTACAACTATTCAATAAATGAGCCGTATTTCCTCCTCCAACAGCAAA
TAATGTGGTCGTCGTTGTCCATAACGTTTAAGTTGTACGCCCATTACTAGCACAAAATAT
ATGAAGAGAAAACATCCATAATGAAAAAGTGATAATAATATGCAAAACATTTATGGATAA
GAAACTGTGATGTAAATGGCTGATCAGCAATTACATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11