Sequences from confirmation amplicon


Confirmation sequences for line 196C12 and hit At1g26560 are shown.
Line ID 196C12
Locus At1g26560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9180602
Primers used for PCR 08ny - 8474, show primer details
   
Sequence Name 31-K114669-0022-196-C12C-08ny
GenBank Accession LN500273 [GenBank]
Sequencing primer gene specific primer, 08ny: 5'-GTTCCTTGCGTAGTACGTTGTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114669-0022-196-C12C-08ny
TGTAAACACCGGTAATCTGCTTCCGACTCTGCTTCTCATCGAGCTTGGGTAATCCCCAAA
CATCAACGGATCCAAAAACCTAATTAAGATTGTTTAAAGATATATGATCACTTGTTATAA
GTTATGGAACAGAGCTAAGTTGTGTAAGAACATATACGTACCAGCCAAGTTGAAAGTCTT
GAGCTCTTTGTGCAGCTTCAATGTCCTCTGTCTTGTTCGACTCTGGTTCAAACCACATTA
CATCAAACGCAATCCCTAACGAACCTCCTTGCTTTGCCTATCATAATTATACATCAAAAC
TACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 08-K114668-0022-196-C12C-8409
GenBank Accession LN489837 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114668-0022-196-C12C-8409
AATTATGATAGGCAAAGCAAGGAGGTTCGTTAGGGATTGCGTTTGATGTAATGTGGTTTG
AACCAGAGTCGAACAAGACAGAGGACATTGAAGCTGCACAAAGAGCTCAAGACTTTCAAC
TTGGCTGGTACGTATATGTTCTTACACAACTTAGCTCTGTTCCATAACTTATAACAAGTG
ATCATATATCTTTAAACAATCTTAATTAGGTTTTTGGATCCGTTGATGTTTGGGGATTAC
CCAAGCTCGATGAGAAGCAGAGTCGGAAGCAGATTACCGGTGTTTACAGGATCTCAATCT
TCTCTGGTGAAGGGTTCTTTAGATTTTGTAGGGATTAATCACTACACAACGTAC

Last Updated on 06.12.2018 16:11