Sequences from confirmation amplicon


Confirmation sequences for line 196F06 and hit At5g19360 are shown.
Line ID 196F06
Locus At5g19360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6522722
Primers used for PCR MJ51 - 8474, show primer details
   
Sequence Name 08-K029908-022-196-F06C-8409
GenBank Accession LN489607 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K029908-022-196-F06C-8409
CATTACAATTGAATATATCCTGACATCTCTTATCTTAAAAGCTCCTATATATATTTCATT
GAACTCTTCTTGACATTTTTGTTGTTTTAAAGATCATCCATGGATCAAGGAGGATGGAGA
AGCACCGGATGTTCCTCTTGACAATGCAGTGATGTCTAGGCTCAAGCAGTTCAAAGCAAT
GAACAACTTTAAGAAAGTTGCTTTACGGGTAATTAACATTCTTCTCAAAACCATATATGT
ATTGTAATAAACAATGCGAGTCTTTTGGTCTATATATCTCATTGGCATCATCAACAAACA
GGTGATAGCCGGGTGCTTATCAGAGGAAGAAATCATGGGGTTAAAAGAGATGTTCAAAGG
AATGGACACTGACAACAGTGGAACCATAACTCTTGAGGAACTAAGACAGGGACTTGCTAA
GCAAGGTACAAGGTTAT
   
Sequence Name 08-K029908-022-196-F06C-MJ51
GenBank Accession LN489608 [GenBank]
Sequencing primer gene specific primer, MJ51: 5'-TTCGTATTCGGATAACCTTGTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K029908-022-196-F06C-MJ51
GTATGGTTCCCTGTTGTCAGTGTCCATTCCTTTGAACATCTCTTTTACCCCATGATTTCT
TCCTCTGATAAGCACCCGGCTATCACCTGTTTGTTGATGATGCCAATGAGATATATAGAC
CAAAAGACTCGCATTGTTTATTACAATACATATATGGTTTTGAGAAGAATGTTAATTACC
CGTAAAGCAACTTTCTTAAAGTTGTTCATTGCTTTGAACTGCTTGAGCCTAGACATCACT
GCATTGTCAAGAGGAACATCCGGTGCTTCTCCATCCTCCTTGATCCATGGATGATCTTTA
AAACAACAAAAATGTCAAGAAGAGTTCAATGAAATATATATAGGAGCTTTTAAGATAAGA
GATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11