Sequences from confirmation amplicon


Confirmation sequences for line 196F10 and hit At1g17930 are shown.
Line ID 196F10
Locus At1g17930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6166713
Primers used for PCR 80pq - 8474, show primer details
   
Sequence Name 48-K115187-0022-196-F10C-80pq
GenBank Accession LN506592 [GenBank]
Sequencing primer gene specific primer, 80pq: 5'-AGTAGTAGAATGGCACTTCCCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K115187-0022-196-F10C-80pq
CCGGAGAGTTCCTCCCGGAAAAACGAGAAAAACCATGATGAAGACTTACTGGAAGACATG
AACACAGCAGATGAGGAATGGATGAGACGAAGGGAAAACATCGTGGAGAATGGAGGAGGT
AACGGTGATGAAAGTGAATATATGCAGTGGTTCAATAGCATCACAGTCCCAAAGCTTCAC
AGAGATACAAGTCTCGAAGCTGATATAATGAACGTGGTAAATGGATCATCTTCTTCTTGG
TTATTTATGTGTAATCACCTCGAAATTTCTTCTTATGTCATTGCTGTTTCTTTTGTAGCA
AGCTGCTATACTACAATTTGACGAGGTGGCCTCAACGTTATCATTAGAGGATCTACATCC
AGAGGAGAGAGAAGCAATTGAAGAAGCAGTGATGTCTATGTCCAATGCCTTGAGAGTAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCA
   
Sequence Name 12-K115186-0022-196-F10C-8409
GenBank Accession LN491703 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115186-0022-196-F10C-8409
ATTTCATTGAATATATCCTACTCTCAAGGCATTGGACATAGACATCACTGCTTCTTCAAT
TGCTTCTCTCTCCTCTGGATGTAGATCCTCTAATGATAACGTTGAGGCCACCTCGTCAAA
TTGTAGTATAGCAGCTTGCTACAAAAGAAACAGCAATGACATAAGAAGAAATTTCGAGGT
GATTACACATAAATAACCAAGAAGAAGATGATCCATTTACCACGTTCATTATATCAGCTT
CGAGACTTGTATCTCTGTGAAGCTTTGGGACTGTGATGCTATTGAACCACTGCATATATT
CACTTTCATCACCGTTACCTCCTCCATTCTCCACGATGTTTTCCCTTCGTCTCATCCATT
CCTCATCTGCTGTGTTCATGTCTTCCAGTAAGTCTTCATCATGGTTTTTCTCGTTTTTCC
TGGGAGGAACTTCTCCGGGGATAACTTGGCACAGCCCAAACTGCTTCATGCATCGATCTG
GGAAGTGCCTNCCTACTAC

Last Updated on 06.12.2018 16:11