Sequences from confirmation amplicon


Confirmation sequences for line 197E08 and hit At1g61140 are shown.
Line ID 197E08
Locus At1g61140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22538537
Primers used for PCR HP15 - 8474, show primer details
   
Sequence Name 43-K017629-022-197-E08C-HP15
GenBank Accession LN504672 [GenBank]
Sequencing primer gene specific primer, HP15: 5'-AACAGCTCTACCTAGCCAAGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017629-022-197-E08C-HP15
ATAAGCTCTCATCATTTTTACGATTCAGATACATGTTTGCAATATGTTGTAGAAGATCCC
AGCCCAGTGACACAAAACAATGAGTATAAAGACTTTCAAATTCAACAAGGAGACCGGGAA
TATATTCAACCGAGGGGCATTGATTCTCAATTCTCAAATGCCAGCTTTGAATCAGTTCAA
AGCCATTCTTCAGAATGTATATCCGATAGTGATGATGATTCTGACGTCTGCATAATAGAA
CCTTATGGTCAATCTGCAATCCCACATCGACCTCTAGCTATGAAAATGCCGGTAGTTTCT
TCAGAATATTCTACAGTTAGTCATAATTTTAATCAATCTGGAGGCCTGAAGCTTCAGTCA
AATAAAGAAAATATGATCTTTCAAGCTGCATTGCAGGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACAT
   
Sequence Name 47-K017629-022-197-E08C-8409
GenBank Accession LN506040 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K017629-022-197-E08C-8409
CATGTAGATTTCCCAGACCTGCAATGCAGCTTGAAAGATCATATTTTCTTTATTTGACTG
AAGCTTCAGGCCTCCAGATTGATTAAAATTATGACTAACTGTAGAATATTCTGAAGAAAC
TACCGGCATTTTCATAGCTAGAGGTCGATGTGGGATTGCAGATTGACCATAAGGTTCTAT
TATGCAGACGTCAGAATCATCATCACTATCGGATATACATTCTGAAGAATGGCTTTGAAC
TGATTCAAAGCTGGCATTTGAGAATTGAGAATCAATGCCCCTCGGTTGAATATATTCCCG
GTCTCCTTGTTGAATTTGAAAGTCTTTATACTCATTGTTTTGTGTCACTGGGCTGGGATC
TTCTACAACATATTGCAAACATGTATCTGAATCGTAAAAATGATTGGAGCTTATCTTGCC
ACTGTCTTGGCTAGG

Last Updated on 06.12.2018 16:11