Sequences from confirmation amplicon


Confirmation sequences for line 197G12 and hit At1g11190 are shown.
Line ID 197G12
Locus At1g11190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3751962
Primers used for PCR 05ea - 8474, show primer details
   
Sequence Name 27-K110359-0022-197-G12C-05ea
GenBank Accession LN498438 [GenBank]
Sequencing primer gene specific primer, 05ea: 5'-AGAGATCTGAGTCATTTGACCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K110359-0022-197-G12C-05ea
AGTCGTAGTGTGTGTTTTTGTGGTGTGACCAGAATCTTTTAGAAGCCGGACCAGCACATG
TAGTAGAGAATCTGTTACCGGATTACGTGAAAGGAGATTTATCAGCATTGTGTGTGTGGC
CTGACCAGATCCGACATTGGTACAAGTATCGTTGGACCAGCCATCTCCATTACATCGACA
CTCCCGACCAAGCCTGCTCTTACGAATACTCTAGTAAGTCACAACCGAGACATTTTCAGA
TAACCTTAATCCGTTTTCTAATTATCTTGAACCGGAGTTAACCAAAAAATCAATTACAAA
TACCAAACCGGATTAAAAACAGGGGATTGTCATGATCAACATGGATTGAAGGATATGTGT
GTGGATATGTGTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAG
   
Sequence Name 11-K110358-0022-197-G12C-8409
GenBank Accession LN491166 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K110358-0022-197-G12C-8409
ATATCCACACACATATCCTTCATCCATGTTGATCATGACAATCCCCTGTTTTTAATCCGG
TTTGGTATTTGTAATTGATTTTTTGGTTAACTCCGGTTCAAGATAATTAGAAAACGGATT
AAGGTTATCTGAAAATGTCTCGGTTGTGACTTACTAGAGTATTCGTAAGAGCAGGCTTGG
TCGGGAGTGTCGATGTAATGGAGATGGCTGGTCCAACGATACTTGTACCAATGTCGGATC
TGGTCAGGCCACACACACAATGCTGATAAATCTCCTTTCACGTAATCCGGTAACAGATTC
TCTACTACATGTGCTGGTCCGGCTTCTAAAAGATTCTGGTCACACCACAAAAACACACAC
ATACGAACTTTTGTTATGAATCTTATAACGGTCAAATGACTCAGATCT

Last Updated on 06.12.2018 16:11