Sequences from confirmation amplicon


Confirmation sequences for line 198E11 and hit At5g54820 are shown.
Line ID 198E11
Locus At5g54820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22270980
Primers used for PCR 09fh - 8474, show primer details
   
Sequence Name 22-K110985-0022-198-E11C-09fh
GenBank Accession LN496166 [GenBank]
Sequencing primer gene specific primer, 09fh: 5'-GTAAAGCTCTACTCTTTCCAACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K110985-0022-198-E11C-09fh
ATCAATACCTCAATACATTTAGCTCGTTTGAGGAACACAAAAGTTCCTAATAACCACGAC
CTTCAGTGTCTTCCTTAGACTCTTACATGTTCTCTTCTGCATCCAATACGTTTTAGGATC
GATGCCTTCATCTGACGAAGTTGCCTACAAAATATTAAAAGAAAATCATGCATATTAAGT
AATGACAAAATGTGACTACTCTTTATTGCATATATATGTAGAAATCTTACCGAGAAAGGA
CATGGAGTTAGAATATCAAAACCAAGCGTCTCCAAATTAGGGCAGTTGTTGAGAAGAAGA
ATTATTCCATTGAACTCCTTGGTATGCATCTTAGTTCTTAACACCAAGTGTCGTGTTTCC
ATAGGTTGAAGCAAATCAGATGGCTTTTCACATTCTGGGATAACCTTCCATAAGAAAAGA
AACACATGTCAATATCAAAAATACATTATAACGGATTAACGGTTAATATATACAACAAAT
TAACGTAGTAGCTGTTATGAATTTAATATAGTAAAACTAAACATTCAAGATGATTTTTAT
ACACTATGTGACTTATTATTGAATAAAATAAACCATCTTCAAAATACTTTAATAAATAAA
CTAAATATTTCAAAAAGTTATCTGGATAAACCTATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 06-K110984-0022-198-E11C-8409
GenBank Accession LN488868 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K110984-0022-198-E11C-8409
ATTGAAAGGTTTATCCGATAACTTTTTGAAATATTTAGTTTATTTATTAAAGTATTTTGA
AGATGGTTTATTTTATTCAATAATAAGTCACATAGTGTATAAAAATCATCTTGAATGTTT
AGTTTTACTATATTAAATTCATAACAGCTACTACGTTAATTTGTTGTATATATTAACCGT
TAATCCGTTATAATGTATTTTTGATATTGACATGTGTTTCTTTTCTTATGGAAGGTTATC
CCAGAATGTGAAAAGCCATCTGATTTGCTTCAACCTATGGAAACACGACACTTGGTGTTA
AGAACTAAGATGCATACCAAGGAGTTCAATGGAATAATTCTTCTTCTCAACAACTGCCCT
AATTTGGAGACGCTTGGTTTTGATATTCTAACTCCATGTCCTTTCTCGGTAAGATTTCTA
CATATATATGCAATAAAGAGTAGTCACATTTTGTCATTACTTAATATGCATGATTTTCTT
TTAATATTTTGTAGGCAACTTCGTCAGATGAAGGCATCGATCCTAAAACGTATTGGATGC
AGAAGAGAACATGTAAGAGTCTAAGGAAGACACTGAAGGTCGTGGTTATTAGGAACTTTT
GTGGTTCCTCAAACGAGCTAAATGTATTGAGGTATTTGATTCGATCAGCGAGCGGTGCTG
GAGATGCGTTGGAAAGAGTAAAGCTTTA

Last Updated on 06.12.2018 16:11