Sequences from confirmation amplicon


Confirmation sequences for line 199G03 and hit At4g26490 are shown.
Line ID 199G03
Locus At4g26490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13380322
Primers used for PCR 15qv - 8474, show primer details
   
Sequence Name 81-K115990-0022-199-G03C-15qv
GenBank Accession LN514109 [GenBank]
Sequencing primer gene specific primer, 15qv: 5'-ATCTGTCCCCTGTAATCAATCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K115990-0022-199-G03C-15qv
AAGCACTTGTTAGAAGAAGGAATATTAAACGAAAACGCATGTAGTTTTCAAACTTTAAAT
CCACCGAGATTTGCTTTACTAGTTCTTCAAAACGACACGCATGGTCCCACTCATTACAAA
AACCAAACCCCATTATGCAAAAGCTCTCTTCTTTTGCCCAAACTCTCGCTTCAAACAAAA
GTTCAATTGAATATACATGAAGACTGGGAAATCTACATTGGCTTCATTGTAAATGGGGTT
TGGACATGAAGCCATTGACGTTATGTGTAATGGTTATGGTTAAAGGTAGATGGTGTTTTT
TGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCA
   
Sequence Name 62-K115989-0022-199-G03C-8409
GenBank Accession LN510832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K115989-0022-199-G03C-8409
TCATTGAATATATCCTGACAAAAACACCATCTACCTTTAACCATAACCATTACACATAAC
GTCAATGGCTTCATGTCCAAACCCCATTTACAATGAAGCCAATGTAGATTTCCCAGTCTT
CATGTATATTCAATTGAACTTTTGTTTGAAGCGAGAGTTTGGGCAAAAGAAGAGAGCTTT
TGCATAATGGGGTTTGGTTTTTGTAATGAGTGGGACCATGCGTGTCGTTTTGAAGAACTA
GTAAAGCAAATCTCGGTGGATTTAAAGTTTGAAAACTACATGCGTTTTCGTTTAATATTC
CTTCTTCTTAACAAGTGGCTTTTCCTAATTAAATCTGAGATAAAAGGTTTTGATTGATTA
CAGGNGACAGATA

Last Updated on 06.12.2018 16:11