Sequences from confirmation amplicon


Confirmation sequences for line 200G05 and hit At1g66740 are shown.
Line ID 200G05
Locus At1g66740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24893105
Primers used for PCR MK79 - 8474, show primer details
   
Sequence Name 78-K101328-0022-200-G05C-8409
GenBank Accession LN513658 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K101328-0022-200-G05C-8409
CTGAATAAAAGCGGTAGTTGCCAACATTAACAGGCCCTACAAGCACACTCTCTAGAAGTT
GATCATAAGTCTCATCTTCTGCTGAGCCTACATAGATAAGCTTCCATTCCAAATCTGTAC
CACAAGCAAAAGCACGGGTCTATCTAAGTTATCCTCACCCTATAACAATGTTAACCAAGA
ACTTGTGCATAGATAAATCCAACTAATAGACAACAAGACTCTAGATTATCAATTAGAAAA
CAAACCATTAGAGCATGACTCTGACAAAGCAATTAGGTAACAATATGTTCCAATTACAAT
CAGCAGCAACAAATTGGTGTTGAAAACAATCCATAAGAATCAGCAGAGACTAAAACCTAA
ATAAAGTCGAGATGAATTACAATTCAGGGGAAGGAGAAAGAGGAGATTTTTACCGTCTTT
GAGAGAATTCAAACACTCGTAAGAAATCTCGAACTGAAAAGGGCTAACAAAAGGAGCA
   
Sequence Name 78-K101334-0022-200-G05C-MK79
GenBank Accession LN513659 [GenBank]
Sequencing primer gene specific primer, MK79: 5'-GCATAATCCTGCTCCTTTTGTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K101334-0022-200-G05C-MK79
TTGATTCTCTCAAGACGGTAAAAATCTCCTCTTTCTCCTTCCCCTGAATTGTAATTCATC
TCGACTTTATTTAGGTTTTAGTCTCTGCTGATTCTTATGGATTGTTTTCAACACCAATTT
GTTGCTGCTGATTGTAATTGGAACATATTGTTACCTAATTGCTTTGTCAGAGTCATGCTC
TAATGGTTTGTTTTCTAATTGATAATCTAGAGTCTTGTTGTCTATTAGTTGGATTTATCT
ATGCACAAGTTCTTGGTTAACATTGTTATAGGGTGAGGATAACTTAGATAGACCCGTGCT
TTTGCTTGTGGTACAGATTTGGAATGGAAGCTTATCTATGTAGGCTCAGCAGAAGATGAG
ACTTATGATCAACTTCTAGAGAGTGTGCTTGTAGGGCCTGTTAATGTTGGCAACTACCGC
TTTGTATTTCAGGTAACATATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA

Last Updated on 06.12.2018 16:11