Sequences from confirmation amplicon


Confirmation sequences for line 200H02 and hit At4g18550 are shown.
Line ID 200H02
Locus At4g18550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10226287
Primers used for PCR BL89 - 8474, show primer details
   
Sequence Name 18-K105393-0022-200-H02C-BL89
GenBank Accession LN494303 [GenBank]
Sequencing primer gene specific primer, BL89: 5'-CTCCAAGAAACCACGATATCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K105393-0022-200-H02C-BL89
CGCCACGTACCCATCCAATTTGATTCCTTTGACCATCCCTCACGTGAAATTGGGAACAAT
AAGAATGACTCAGGCACGTGGATATCGGATGTCGCGTATATAAACTTCGTCACTTTGTAC
TTTGTGTACGGGTGTGCTATCTCTAAACCAACCTGTGTTTTAAGAATTTACAAAAATGCG
ATTTAGGTTTCTTGCTTTACAAGAAAGTACCTTCTCCCTTTAGTTTTGCTAAGAAATAAA
TATTTATTTTACTTCGATTGGTGACATCTACATGGTATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 02-K105394-0022-200-H02C-8409
GenBank Accession LN487030 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K105394-0022-200-H02C-8409
CATTTACATTGAATATACCATGTAGATGTCACCAATCGAAGTAAAATAAATATTTATTTC
TTAGCAAAACTAAAGGGAGAAGGTACTTTCTTGTAAAGCAAGAAACCTAAATCGCATTTT
TGTAAATTCTTAAAACACAGGTTGGTTTAGAGATAGCACACCCGTACACAAAGTACAAAG
TGACGAAGTTTATATACGCGACATCCGATATCCACGTGCCTGAGTCATTCTTATTGTTCC
CAATTTCACGTGAGGGATGGTCAAAGGAATCAAATTGGATGGGTTACGTGGCGGTAACAG
ACGACCAAGGCACGGCACTTCTTGGTCGGAGAGATATCGTGGTTTCTTGGAG

Last Updated on 06.12.2018 16:11