Sequences from confirmation amplicon


Confirmation sequences for line 200H12 and hit At4g39650 are shown.
Line ID 200H12
Locus At4g39650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18403680
Primers used for PCR RH05 - 8474, show primer details
   
Sequence Name 05-K018600-022-200-H12C-RH05
GenBank Accession LN488155 [GenBank]
Sequencing primer gene specific primer, RH05: 5'-GCAAGTTTCTCAGCAGGTTCTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K018600-022-200-H12C-RH05
ACTTCCCGTGTTGTGTCCAAGCCGTGTATAGACCCGCGACTTCCCCGGGAACGCCTACTG
ACAAGGATCCTTTCTTCTTTCGCTCAGGATTATCACCATACATATCCTACATCAAGAGAA
ACAGCCAATAAATATCTTTGATTAAAAAAACACTGTCCATAACTCAAAAACGTACGTTAT
CCAAAATGCTCATTTTGGGAAGAGTAAAAAGAAAGACGTTATTACTGTAAAACGTGTTAC
TTACCTCAGTGGCGCTTAGAGGAGCGGTTTCTCTGGAATCATAAGCAACTTCCGTGCCAT
TAGCTAGCTTAATCATTGTAAACGCTCCACCGCCTATACCGCTAGATGCGGGACTGACAA
CGCCCAAACAAAGAGCAGCAGCCACCGACGCATCAATCGCGTTTCCTCCTTGACGAAGAA
CATTCGTCCCTATTGCAGAACACCGTCCATCGTCTGTCGCAACCGCACCGTGATACGCAA
CAATACTTTGCAGATTTTTCACAGCGTTAGCGTTTTGCAGAAACGCGATTAGAAGAAGAG
CGATCGTCGCAGTACGAACGAGCGACATAAATGCTACGATCAATAGAGAATGAAACCAGA
GAGATTAATCAGCAATGAGTATGATGGTCATATGGAGGAANAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCANAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACG
   
Sequence Name 06-K018600-022-200-H12C-8409
GenBank Accession LN488601 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018600-022-200-H12C-8409
TAATCTCTCTGGTTTCATTCTCTATTGATCGTAGCATTTATGTCGCTCGTTCGTACTGCG
ACGATCGCTCTTCTTCTAATCGCGTTTCTGCAAAACGCTAACGCTGTGAAAAATCTGCAA
AGTATTGTTGCGTATCACGGTGCGGTTGCGACAGACGATGGACGGTGTTCTGCAATAGGG
ACGAATGTTCTTCGTCAAGGAGGAAACGCGATTGATGCGTCGGTGGCTGCTGCTCTTTGT
TTGGGCGTTGTCAGTCCCGCATCTAGCGGTATAGGCGGTGGAGCGTTTACAATGATTAAG
CTAGCTAATGGCACGGAAGTTGCTTATGATTCCAGAGAAACCGCTCCTCTAAGCGCCACT
GAGGTAAGTAACACGTTTTACAGTAATAACGTCTTTCTTTTTACTCTTCCCAAAATGAGC
ATTTTGGATAACGTACGTTTTTGAGTTATGGACAGTGTTTTTTTAATCAAAGATATTTAT
TGGCTGTTTCTCTTGATGTAGGATATGTATGGTGATAATCCTGAGCGAAAGAAGAAAGGA
TCCTTGTCAGTAGGCGTTCCCGGGGAAGTCGCGGGTCTATACACGGCTTGGACACAACAC
GGGAAGTTACCGTGGAAGCAGTTAGTAGAACCTGC

Last Updated on 06.12.2018 16:11