Sequences from confirmation amplicon


Confirmation sequences for line 201A01 and hit At4g04890 are shown.
Line ID 201A01
Locus At4g04890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2478741
Primers used for PCR AY09 - 8474, show primer details
   
Sequence Name 27-K016209-022-201-A01C-8409
GenBank Accession LN498160 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K016209-022-201-A01C-8409
TAGATTTCCCGGACATGAATCAAAAGATTAAAACTTTAGTTAGTTTATTCAATCATTTTT
AGCCCTTAAGTTACTTGAAGAACAAGTAATTACCACATCCATGAGAATCTCAACGAGATT
GATGTGATTCATTATAACAACTGCAGATTGTCTTGACGCCTCTGATCTTAATCCTAATGG
CTTTGGTCCAATTCCTCTCGGAAACGTTCTGAAATACTCTTCTTCGTTGAGAATCTCGAC
TGAATTATCGGTTGAAAGCCATAAAGGATCTCCAGTTTGAGCCATTCTCACGAGTTCCTC
CATAGCTGCAACCGCTAGCTCCACGATTATAGGCTTATCAGTCTCAGAAGGAATCGAAAC
TG
   
Sequence Name 28-K016209-022-201-A01C-AY09
GenBank Accession LN498626 [GenBank]
Sequencing primer gene specific primer, AY09: 5'-GTCAGTTTCGATTCCTTCTGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K016209-022-201-A01C-ay09
ATAAGCCTATAATCGTGGAGCTAGCGGTTGCAGCTATGGAGGAACTCGTGAGAATGGCTC
AAACTGGAGATCCTTTATGGCTTTCAACCGATAATTCAGTCGAGATTCTCAACGAAGAAG
AGTATTTCAGAACGTTTCCGAGAGGAATTGGACCAAAGCCATTAGGATTAAGATCAGAGG
CGTCAAGACAATCTGCAGTTGTTATAATGAATCACATCAATCTCGTTGAGATTCTCATGG
ATGTGGTAATTACTTGTTCTTCAAGTAACTTAAGGGCTAAAAATGATTGAATAAACTAAC
TAAAGTTTTAATCTTTTGATTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATTC

Last Updated on 06.12.2018 16:11