Sequences from confirmation amplicon


Confirmation sequences for line 203G03 and hit At4g33240 are shown.
Line ID 203G03
Locus At4g33240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16036162
Primers used for PCR 12vh - 8474, show primer details
   
Sequence Name 17-K117806-0022-203-G03C-12vh
GenBank Accession LN494043 [GenBank]
Sequencing primer gene specific primer, 12vh: 5'-CTTAAGAGATTTGCATGATGTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117806-0022-203-G03C-12vh
GGTCTTTCAATGATTCACTATTGTCTCAATTTGAATTTATTAGTTGAGTTGCTCATTTTC
GTGATTAGTAGTGGCAGGAGTGATGGTGAAGCTGATGACGATGACGATTATCAATCTGAT
TTTGCACAATCTTACGCTCAAGGAAATGACTACTATGGTGCCATCAACCTCGATGAAGTA
GACCATATCTATGGGTCACATGAAGCTCATGACGTTGGTGTAAAAATAGAACCAAACATA
AGTGGCTTTCCCCCTGATCAAGATCTTGACTCACTGAATACAGAGACGATAGATAAAACC
AGACAACAAGAAAATGGATGGAACGATGTAAAGGAGGGAAGCCCTCCTTGTGAAGAGTCC
TTTGAGCCCGAAGTTGTTGATTTTGAGAGTGATGGGCTCTTATGGCTACCACTGATTTGG
TAGATTTTGCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATATCCACA
   
Sequence Name 17-K117784-0022-203-G03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117784-0022-203-G03C-8409
AACTCATCTAACAAATTCGAATTAAGACGATAATGAATCATTTGAAAAGACCCAGTACAG
CAAATACACTTGGTGATGGACATAATGCAAATGTCAAAAGAGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATA

Last Updated on 06.12.2018 16:11