Genotyping and primer details


Genotyping and primer details for 203G03 - At4g33240
Line ID 203G03
Locus At4g33240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16036162 of line 203G03 Chr4:16036162
   
Insertion allele amplicon 8474 - 12vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12vh: 5'-CTTAAGAGATTTGCATGATGTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16036623
   
wt amplicon (over InsPos) 31vq - 12vh
wt amplicon start Chr4:16035365
wt amplicon end Chr4:16036623
wt amplicon size 1259 bp
Locus specific primer 12vh: 5'-CTTAAGAGATTTGCATGATGTCCA-3'
2nd locus specific primer 31vq: 5'-ACAAGGAGATAAAGCTCCAAATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11