Sequences from confirmation amplicon


Confirmation sequences for line 203H06 and hit At2g03480 are shown.
Line ID 203H06
Locus At2g03480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1053285
Primers used for PCR 13uh - 8474, show primer details
   
Sequence Name 42-K117093-0022-203-H06C-13uh
GenBank Accession LN504611 [GenBank]
Sequencing primer gene specific primer, 13uh: 5'-TAAGCTTGGCTTTCCTGTATTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117093-0022-203-H06C-13uh
CGCAGCTTCTATACCTCTTTGCAAGATGGAGATAGCGTTCCGTATTACCACCCATTGGTT
CCATGTATAAGCGGAACCACGAGTAAACGCTGGATTTCTATACAGAACAGGTCTGCTGTT
GCAGGAACAACCTCTGCCGGGCTTGAAATTCATGGAAAGTATTTCTTTGGATAACCACTT
CGTTGTTTCTTTCTCATTGTTTAAATTTTACTTGATACTTTCAGAATTTAATATGTTGTT
TTGGATATACTGAATACAGGTTTAAAACCGGAAGAATTCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTAT
   
Sequence Name 26-K117092-0022-203-H06C-8409
GenBank Accession LN498101 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117092-0022-203-H06C-8409
CTTCCGGTTTTAACCTGTATTCAGTATATCCAAAACAACATATTAAATTCTGAAAGTATC
AAGTAAAATTTAAACAATGAGAAAGAAACAACGAAGTGGTTATCCAAAGAAATACTTTCC
ATGAATTTCAAGCCCGGCAGAGGTTGTTCCTGCAACAGCAGACCTGTTCTGTATAGAAAT
CCAGCGTTTACTCGTGGTTCCGCTTATACATGGAACCAATGGGTGGTAATACGGAACGCT
ATCTCCATCTTTGCAAAGAGGTATAGAAGCTTGCGAACTAGACCAACCAGAAAAGAAAGA
AAAAGCATCAATACAGGAAAGNCAAGCTT

Last Updated on 06.12.2018 16:11